摘要
目的观察矢车菊素-3-O-葡萄糖苷(C3G)对人原代成骨细胞增殖作用及对c-Jun氨基末端激酶/叉头盒蛋白O1(JNK/FOXO1)的影响。方法收集2017年6月至2018年6月沈阳医学院附属中心医院60~90周岁的骨质疏松且股骨颈或股骨粗隆间骨折行人工股骨头或全髋置换的患者的松质骨组织,分离培养体外成骨细胞。MTT法检测control组(含0μmol/L C3G的α-MEM培养液)、C3G组(含100μmol/L C3G的α-MEM培养液)人原代成骨细胞细胞的增殖活力;实时荧光定量PCR法检测control组和C3G组FOXO1、转录激活因子-4(ATF4)和骨保护素(OPG)mRNA表达的情况。使用JNK通路抑制剂(SP600125)对细胞进行处理,实时荧光定量PCR法检测SP600125-control组和SP600125+C3G组FOXO1 mRNA表达的情况。结果C3G组人原代成骨细胞增殖率高于control组,差异有统计学意义(P<0.05)。C3G组人原代成骨细胞FOXO1、ATF4和OPG mRNA表达量低于control组,差异有统计学意义(P<0.05)。SP600125-control组和SP600125-C3G组FOXO1的mRNA相对表达量比较,差异无统计学意义(P>0.05)。结论C3G可能通过JNK通路影响FOXO1基因水平促进人原代成骨细胞增殖。
Objective To observe the effect of cyanidin-3-O-glucoside(C3G)on the proliferation of human primary osteoblasts and its effect on c-Jun N-terminal kinase/forkhead box O1(JNK/FOXO1).Methods The cancellous bone tissues were collected from 60-90 years old patients with osteoporosis and femoral neck or intertrochanteric fracture who underwent artificial femoral head replacement or total hip replacement in Central Hospital Affiliated to Shenyang Medical College from June 2017 to June 2018,and osteoblasts were isolated and cultured in vitro.MTT assay was used to detect the proliferation activity of human primary osteoblasts in control group(α-MEM medium containing 0μmol/L C3G),C3G group(α-MEM medium containing 100μmol/L C3G).Real-time fluorescence quantitative PCR was used to detect mRNA expression of FOXO1,activiating transcripition factor-4(ATF4),and osteoprotegerin(OPG)in control group and C3G group.Cells were treated with JNK pathway inhibitor(SP600125),and FOXO1 mRNA expression in SP600125-control group and SP600125-C3G group were detected by real-time fluorescence quantitative PCR.Results The proliferation rate of human primary osteoblasts in C3G group was higher than that in control group,and the difference was statistically significant(P<0.05).The mRNA expression of FOXO1,ATF4,and OPG in C3G group were decreased than those in control group,and the differences were statistically significant(P<0.05).There were no significant differences in the mRNA expression of FOXO1 between SP600125-control group and SP600125-C3G group(P>0.05).Conclusion C3G may affect FOXO1 gene level through JNK pathway to promote human primary osteoblasts proliferation.
作者
陈琳
胡博森
周波
陈拥
CHEN Lin;HU Bosen;ZHOU Bo;CHEN Yong(School of Public Health,Shenyang Medical College,Liaoning Province,Shenyang110034,China;Department of Orthopedics,Central Hospital Affiliated to Shenyang Medical College,Liaoning Province,Shenyang110024,China)
出处
《中国医药导报》
2021年第23期18-21,共4页
China Medical Herald
基金
辽宁省自然科学基金计划重点项目(20170540879)
辽宁省沈阳市科技计划项目(19-112-4-024)
沈阳医学院科技基金项目(20182045)
沈阳医学院硕士研究生科技创新基金项目(Y20190508)。
