摘要
目的探讨红景天苷激活PI3K/AKT通路改善绝经后骨质疏松症的作用。方法建立绝经后骨质疏松症大鼠模型,随机分为模型组、LY294002(PI3K/Akt通路抑制剂)组、红景天苷组、红景天苷+LY294002组,每组12只,另取12只设为假手术组。分组处理后,通过骨科生物力学测试仪测定大鼠股骨生物力学指标弹性模量、最大载荷、屈服载荷;测定大鼠股骨骨矿盐含量;运用苏木精-伊红(HE)染色检测各组大鼠骨组织病理变化;通过酶联免疫吸附法(ELISA)检测血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平;运用蛋白免疫印迹法检测脑组织PI3K/Akt通路相关蛋白表达情况。结果与假手术组相比,模型组大鼠骨组织呈现骨小梁稀疏断裂、数目明显减少,有较多空隙、不能连接成网等病理损伤,血清IL-6及TNF-α水平均显著升高(P<0.05),股骨弹性模量、最大载荷、屈服载荷、骨矿盐含量、骨组织p-PI3K/PI3K、p-Akt/Akt水平均显著降低(P<0.05);与模型组相比,红景天苷组大鼠骨组织病理损伤减轻,血清IL-6及TNF-α水平降低(P<0.05),股骨弹性模量、最大载荷、屈服载荷、骨矿盐含量、骨组织p-PI3K/PI3K、p-Akt/Akt水平升高(P<0.05);LY294002组大鼠骨组织病理损伤加重,血清IL-6及TNF-α水平升高(P<0.05),股骨弹性模量、最大载荷、屈服载荷、骨矿盐含量、骨组织p-PI3K/PI3K、p-Akt/Akt水平降低(P<0.05)。与LY294002组相比,红景天苷+LY294002组大鼠骨组织病理损伤减轻,血清IL-6及TNF-α水平降低(P<0.05),股骨弹性模量、最大载荷、屈服载荷、骨矿盐含量、骨组织p-PI3K/PI3K、p-Akt/Akt水平升高(P<0.05)。与红景天苷组相比,红景天苷+LY294002组大鼠骨组织病理损伤加重,血清IL-6及TNF-α水平升高(P<0.05),股骨弹性模量、最大载荷、屈服载荷、骨矿盐含量、骨组织p-PI3K/PI3K、p-Akt/Akt水平降低(P<0.05)。结论红景天苷可能通过激活PI3K/Akt通路改善绝经后骨质疏松症。
Objective To investigate the effect of salidroside on postmenopausal osteoporosis by activating PI3K/Akt pathway.Methods The rat model of postmenopausal osteoporosis was established and randomly divided into model group,LY294002(PI3K/Akt pathway inhibitor)group,salidroside group and salidroside+LY294002 group,with 12 in each group,another 12 were set as sham operation group.After treatment,the elastic modulus,the maximum load and the yield load of the biomechanical index of the femur of the rats were measured with the orthopedic biomechanical tester;the mineral salt content of femur was measured;hematoxylin eosin(HE)staining was used to detect the pathological changes of bone tissue;the levels of serum IL-6 and TNF-αwere detected by enzyme-linked immunosorbent assay(ELISA);and the expression of PI3K/Akt pathway related proteins was detected by Western blot.Re sults Compared with the sham operation group,the bone tissue of the model group showed sparsely broken trabeculae,the number of trabeculae was significantly reduced,and there were many spaces,unable to connect into nets and other pathological damages,the levels of serum IL-6 and TNF-αincreased significantly(P<0.05),while the elastic modulus,maximum load,yield load of femur,mineral salt content of bone,and levels of p-PI3K/PI3K and p-Akt/Akt in bone tissue decreased significantly(P<0.05);compared with the model group,the pathological injury of bone tissue in salidroside group was reduced,the levels of serum IL-6 and TNF-αdecreased(P<0.05),while the elastic modulus,maximum load,yield load of femur,mineral salt content of bone,and levels of p-PI3K/PI3K and p-Akt/Akt in bone tissue increased significantly(P<0.05);in LY294002 group,the pathological injury of bone tissue was aggravated,the levels of serum IL-6 and TNF-αwere significantly increased(P<0.05),while the elastic modulus,maximum load,yield load of femur,mineral salt content of bone,and levels of pPI3K/PI3K and p-Akt/Akt in bone tissue decreased significantly(P<0.05).Compared with LY294002 group,the pathological injury of bone tissue in salidroside+LY294002 group was reduced,the levels of serum IL-6 and TNF-αwere significantly decreased(P<0.05),while the elastic modulus,maximum load,yield load of femur,mineral salt content of bone,and levels of p-PI3K/PI3K and p-Akt/Akt in bone tissue increased significantly(P<0.05).Compared with salidroside group,the pathological injury of bone tissue in salidroside+LY294002 group was aggravated,the levels of serum IL-6 and TNF-αwere significantly increased(P<0.05),while the elastic modulus,maximum load,yield load of femur,mineral salt content of bone,and levels of p-PI3K/PI3K and p-Akt/Akt in bone tissue decreased significantly(P<0.05).Conclusion Salidroside may improve postmenopausal osteoporosis by activating PI3K/Akt pathway.
作者
黄永湘
李强
李宝林
陶永亮
HUANG Yongxiang;LI Qiang;LI Baolin;TAO Yongliang(Department of Orthopedics of Danzhou People’s Hospital,Danzhou 571700;Department of Orthopedics,Affiliated Hospital of Hainan Medical College,Haikou 570216,China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2021年第8期1135-1139,1146,共6页
Chinese Journal of Osteoporosis
基金
海南省自然科学基金项目(817326)。
