摘要
建立了同时测定人血浆中11种抗癫痫类药物的超高效液相色谱-质谱检测(UPLC-MS/MS)法。血浆样品经甲醇沉淀后进样分析,采用多反应监测(MRM)模式,结合正、负离子切换分段扫描分析。结果表明,11种抗癫痫类药物,乙酰唑胺、扑米酮、唑尼沙胺、加巴喷丁、左乙拉西坦、普瑞巴林、拉莫三嗪、布瓦西坦、丙戊酸钠、地西泮和硝西泮,在相应浓度范围内线性关系良好,最低检测限分别为0.53、0.10、0.11、0.50、0.50、2.06、0.10、0.10、498、0.10、0.10 ng/ml。日内、日间精密度RSD均小于15%;提取回收率均大于70%。建立的方法灵敏、快速、准确、专属性强,可用于上述11种抗癫痫类药物的血药浓度测定及药代动力学研究。
An UPLC-MS/MS method was established for the determination of eleven antiepileptic drugs in human plasma.Plasma samples were precipitated with methanol and then injected for analysis.Multiple reaction monitoring(MRM)mode was performed combined with the positive and negative ion switching and segmented scanning analysis.The results showed that it was linear for the eleven antiepileptic drugs,including acetazolamide,primidone,zonisamide,gabapentin,levertiracetam,pregabalin,lamotrigine,brivaracetam,sodium valproate,diazepam and nirazepam,in the corresponding ranges,and the LLODs were 0.53,0.10,0.11,0.50,0.50,2.06,0.10,0.10,498,0.10 and 0.10 ng/ml,respectively.The intra-day and inter-day precision RSDs were both less than 15%,and the extraction recoveries were greater than 70%.The established method is sensitive,rapid,accurate,and highly specific,which can be used for the blood concentration determination and pharmacokinetic study of the eleven antiepileptic drugs.
作者
王伟
徐唯哲
李清艳
崔玉静
刘国如
WANG Wei;XU Weizhe;LI Qingyan;CUI Yujing;LIU Guoru(Civil Aviation General Hospital,Civil Aviation Medical Center,Civil Aviation Administration of China,Beijing 100123)
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2021年第7期948-954,共7页
Chinese Journal of Pharmaceuticals
基金
民航空勤人员和空管人员违禁药物监管体系建设研究(2020年度民航安全能力建设项目)
事故调查空中失能性疾病药物技术开发研究(2021年度民航安全能力建设项目)。
