摘要
目的:探讨红景天苷在抗骨质疏松症中的作用及其可能机制。方法:培养小鼠骨髓间充质干细胞(BMSCs),用红景天苷加以处理,用PBS加以处理作为对照组,采用碱性磷酸酶(ALP)活性实验和茜素红染色检测两组BMSCs成骨分化能力,并采用实时PCR和蛋白印迹实验检测两组成骨分化相关基因(ALP和BMP-2)的mRNA和蛋白表达情况。用去卵巢法建立骨质疏松症小鼠模型,灌胃给予红景天苷药物,灌胃给予生理盐水作为对照组。分别检测两组小鼠股骨的抗弯能力和骨小梁面积。结果:红景天苷组ALP活性(0.944±0.132)高于对照组(0.714±0.134),P<0.05;茜素红染色结果显示红景天苷组细胞矿化程度明显高于对照组;量化两组矿化钙结节结果显示红景天苷组钙结节形成数目(24.40±5.20)高于对照组(16.55±4.40),P<0.05。在小鼠BMSCs成骨分化基因ALP、BMP-2的mRNA相对表达量方面,红景天苷组均高于对照组。在小鼠BMSCs成骨分化基因ALP、Runx2的蛋白相对表达量方面,红景天苷组均高于对照组。与对照组相比,红景天苷组小鼠股骨抗弯强度(832.95±65.36)N/mm高于对照组(776.39±41.64)N/mm;microCT检测结果表明,红景天苷组小鼠股骨下段骨小梁面积(21.68±4.89)μm^(2)大于对照组(16.47±3.65)μm^(2)。结论:红景天苷能一定程度上促进干细胞的成骨分化能力,动物实验证实红景天苷有很好的抗小鼠骨质疏松症的作用,其作用机制与ALP和BMP-2的表达相关。
Objective:To investigate the role of salidroside in osteoporosis and its possible mechanism.Methods:Mouse bone marrow mesenchymal stem cells(BMSCs)were cultured and treated with salidroside and PBS as control group.ALP activity test and alizarin red staining were used to detect the osteogenic differentiation ability of BMSCs in two groups.The expression of osteogenic differentiation related genes(ALP and BMP-2)was detected by RT-PCR and Western Blotting.Osteoporosis mouse model was established by ovariectomy.Salidroside was administered intragastrically and saline was used as control group.The femoral bending resistance and trabecular bone volume of the two groups of mice were measured respectively.Results:The ALP activity of salidroside group(0.944±0.132)was higher than that in the control group(0.714±0.134)(P<0.05).Alizarin red staining showed that salidroside group had higher cellular mineralization than that in the control group.Quantitative analysis of mineralized calcium nodules showed that the number of calcium nodules in the salidroside group(24.40±5.20)was higher than that in the control group(16.55±4.40)(P<0.05).The relative expression levels of osteogenic differentiation genes ALP and BMP-2 in the BMSCs of the salidroside group were higher than those in the control group.The relative expression levels of osteogenic differentiation genes ALP and Runx2 in the BMSCs of the salidroside up-regulated group were higher than those in the control group.Compared with the control group,the flexural strength of the femur in the salidroside group(832.95±65.36)N/mm was higher than that in the control group(776.39±41.64)N/mm,and the microCT scan showed that the area of trabecula in the lower femur of salidroside group mice(21.68±4.89)μm^(2) were larger than those in the control group(16.47±3.65)μm^(2).Conclusion:Salidroside can promote the osteogenic differentiation of stem cells.Animal experiments had confirmed that salidroside could improve the degree of osteoporosis in mice,and its mechanism was related to ALP and BMP-2.
作者
刘雷
张明焕
陶圣祥
毛文
LIU Lei;ZHANG Minghuan;TAO Shengxiang;MAO Wen(Department of Orthopaedics,the Third Hospital of Wuhan,Wuhan 430060,China)
出处
《中国中医骨伤科杂志》
CAS
2021年第8期18-21,共4页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
武汉市卫健委面上项目(WX19C18)。
关键词
骨质疏松症
骨髓间充质干细胞
红景天苷
成骨分化
osteoporosis
bone marrow mesenchymal stem cells
salidroside
osteogenic differentiation
