摘要
目的:建立金樱根基原之一的粉团蔷薇根质量控制标准。方法:性状、显微、TLC法进行定性鉴别;根据2020年版《中华人民共和国药典》方法进行检查和浸出物测定;UPLC测定粉团蔷薇根中构莓苷F1、阿江榄仁亭、野蔷薇苷含量,并以UPLC建立指纹图谱。结果:所建立的显微鉴别、薄层色谱法、UPLC含量测定和指纹图谱方法稳定可行。药材水分、总灰分及浸出物含量:水分不得过13.87%,总灰分不得过8.72%,浸出物不得少于5.08%;11个批次粉团蔷薇根中所测构莓苷F1、阿江榄仁亭、野蔷薇苷含量范围分别是0.44-12.45、0.32-3.79、1.10^8.08 mg·g^(-1),暂定本品按干燥品计算,含构莓苷F1不得少于0.35 mg·g^(-1),含阿江榄仁亭不得少于0.26 mg·g^(-1),含野蔷薇苷不得少于0.88 mg·g^(-1);同时测定了11批粉团蔷薇根药材的指纹图谱并建立了共有模式,标定共有峰15个,指认了其中的构莓苷F1、阿江榄仁亭、野蔷薇苷、蔷薇酸、委陵菜酸5个成分。结论:该研究所建方法专属性强,可从整体上对粉团蔷薇根进行鉴别和质量控制,也为最终制定金樱根药材质量标准打下基础。
Objective:To establish the quality standard of the roots of Rosa multiflora Thunb.var.cathayensis Rehd.&Wils.Methods:Qualitative identification was carried out by character,microscope and TLC.Examination and extract were determined according to Chinese Pharmacopoeia(2020 edition).The contents of kaji-ichigoside F1,arjunetin and rosamultin were determined by UPLC,and the fingerprints were also established by UPLC.Results:The methods of TLC and UPLC were stable and feasible.The moisture content in the sample should not exceed 13.87%,the total amount of ash should not be more than 8.72%,and the content of extracts should not be less than 5.08%.The contents of kaji-ichigoside F1,arjunetin and rosamultin in 11 batches of samples were 0.44-12.45 mg·g^(-1),0.32-3.79 mg·g^(-1) and 1.10-8.08 mg·g^(-1),respectively.And the minimum contents of the three components should not be less than 0.35 mg·g^(-1),0.26 mg·g^(-1) and 0.88 mg·g^(-1) respectively.The common mode was established after determing the fingerprints of 11 batches from R.multiflora var.cathayensis by UPLC.There were 15 commom peaks in these samples,and five components(kaji-ichigoside F1,arjunetin,rosamultin,euscaphic acid and tormentic acid)were identified.Conclusion:The methods are specific and can be used to identify and control the quality of the roots of Rosa multiflora Thunb.var.cathayensis Rehd.&Wills in general,which also laid a solid foundation for the final formulation of standards for Radix et Caulis Rosae.
作者
邓巧玉
李宇璐
马国需
许旭东
罗轶
周艳林
钟小清
易蔚
袁经权
DENG Qiao-yu;LI Yu-lu;MA Guo-xu;XU Xu-dong;LUO Yi;ZHOU Yan-lin;ZHONG Xiao-qing;YI Wei;YUAN Jing-quan(Guangxi University of Chinese Medicine,School of Pharmacy&Science Experimental Center,Nanning 530200,China;Chinese Academy of Medical Sciences,Institute of Medicinal Plant Development,Beijing 100193,China;Guangxi Institute for Food and Drug Control,Nanning,530021,China;Guilin Sanjin Pharmaceutical Company Limited,Guilin 541004,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2021年第7期1125-1132,共8页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金资助项目(81360683)
广西中医药大学博士科研启动基金资助项目(2018BS048)
广西中医药大学研究生教育创新计划资助项目(YCSY20190029)。
关键词
粉团蔷薇根
构莓苷F1
阿江榄仁亭
野蔷薇苷
蔷薇酸
委陵菜酸
薄层色谱鉴别
含量测定
指纹图谱
质量控制
the roots of Rosa multiflora Thunb.var.cathayensis Rehd.&Wills
kaji-ichigoside F1
arjunetin
rosamultin
euscaphic acid
tormentic acid
TLC
content determination
fingerprinting
quantitative control
