摘要
目的:对比应用实时荧光核酸恒温扩增(simultaneous amplification and testing,SAT)-RNA(SAT-RNA法)与应用聚合酶链式反应(polymerase chain reaction,PCR)-DNA(PCR-DNA法)检测男性生殖道沙眼衣原体、解脲支原体的效果,以探讨SAT-RNA法的应用价值。方法:收集2016年4月至2017年4月于中国医科大学附属盛京医院生殖医学中心就诊的因女方因素拟行体外受精-胚胎移植助孕的163例男性,采用SAT-RNA法检测尿液标本中的沙眼衣原体、解脲支原体,并对其中109例符合当天采集精液标本条件者进行相应病原体的检测。同时采用PCR-DNA法检测163例男性尿道拭子标本中的相应病原体。结果:163例男性生殖道解脲支原体检测结果表明,PCR-DNA法尿道拭子检测阳性77例(阳性率47.24%),SAT-RNA法尿液标本检测阳性78例(阳性率47.85%),两者差异无统计学意义(χ^(2)=0,P>0.05),两种方法的检测结果符合率为93.25%,阳性符合率93.51%,阴性符合率93.02%,检验结果一致性极好(Kappa值0.865)。163例男性生殖道沙眼衣原体检测结果表明,PCR-DNA法尿道拭子检测阳性5例(阳性率3.07%),SAT-RNA法尿液标本检测阳性7例(阳性率4.29%),两者差异无统计学意义(χ^(2)=0.25,P>0.05),两种方法检测结果符合率为97.55%,阳性符合率80.00%,阴性符合率98.10%,检验结果一致性较好(Kappa值0.654)。对其中109例男性采用SAT-RNA法同时检测尿液和精液标本中的解脲支原体和沙眼衣原体:(1)解脲支原体:尿液标本阳性55例(阳性率50.46%),精液标本阳性49例(阳性率44.95%),两标本差异无统计学意义(χ^(2)=2.08,P>0.05),检测结果符合率为88.99%,阳性符合率93.88%,阴性符合率85.00%,检验结果一致性较好(Kappa值0.780);(2)沙眼衣原体:尿液标本阳性6例(阳性率5.50%),精液标本阳性4例(阳性率3.67%),两标本差异无统计学意义(χ^(2)=0.5,P>0.05),检测结果符合率为98.17%,阳性符合率100.00%,阴性符合率98.10%,检验结果一致性较好(Kappa值0.791)。结论:SAT-RNA法与PCR-DNA法检测生殖道沙眼衣原体、解脲支原体有良好的一致性;相比PCR-DNA法,SAT-RNA法可用于尿液或精液标本的检测,具有无创、方便等优势,更适宜临床应用。
Objective:To investigate the value of clinical application of simultaneous amplification and testing of RNA(SAT-RNA)for detecting Chlamydia trachomatis(CT)and Ureaplasma urealyticum(UU)by comparing with the polymerase chain reaction testing of DNA(PCR-DNA)method.Methods:Specimens from both urethra swab and the first avoid urine which should be at least one hour after the previous urination were collected from 163 men who were scheduled for in vitro fertilization and embryo transfer(IVF-ET)treatment due to female factors at Center for Reproductive Medicine,Shengjing Hospital of China Medical University during the period of April 2016 to April 2017.Among the 163 men,109 simultaneously provided semen that was collected after 3-7 days of sexual abstinence for the testing.Urine and semen specimens were detected for CT and UU with SAT-RNA,while urethra swab specimens were detected for CT and UU with standard PCR-DNA.Detection results of the SAT-RNA were compared with those of the PCR-DNA method.Results:The positive rate of UU in the urethra swab detected with PCR-DNA and that of UU in the urine with SAT-RNA were 47.24%and 47.85%,respectively,and the coincidence rate was 93.25%.In addition,the positive and negative coincidence rates were 93.51%and 93.02%,respectively,and the concordance between the two methods was very good(Kappa=0.865).On the other hand,the positive rate of CT in the swab specimen tested with PCR-DNA was 3.07%and that of CT in urine with SAT-RNA was 4.29%,and the coincidence rate was 97.55%.Moreover,the positive and negative coincidence rates were 80.00%and 98.10%,respectively,and the concordance between the two methods was good(Kappa=0.654).Regarding SAT-RNA detection of UU in the urine and semen specimen of the 109 patients,the positive rates of UU in the urine and semen specimens were 50.46%and 44.95%,respectively;and the coincidence rate between the two specimens was 88.99%.In addition,the positive coincidence rate and the negative coincidence rate was 93.88%and 85.00%,respectively,and the concordance between the two specimens was good(Kappa=0.780).Similarly,SAT-RNA detection of CT in the urine and semen specimens showed the positive rate was 5.50%and 3.67%,respectively;and the two specimens showed 98.17%coincidence rate.The positive and negative coincidence rates were 100.00%and 98.10%,respectively,and the concordance was also good(Kappa=0.791).Conclusion:SAT-RNA detection of CT and UU in the urine specimen showed good concordance with the PCR-DNA detection of CT and UU in the urethra swab specimen.In addition,the concordance was also good between the urine and semen specimens detected with SAT-RNA.These results indicate that,as a less invasive and equally accurate procedure,SAT-RNA may be more suitable for clinical application.
作者
杜强
洪锴
潘伯臣
DU Qiang;HONG Kai;PAN Bo-chen(Center for Reproductive Medicine,Shengjing Hospital of China Medical University,Shenyang 110004,China;Department of Urology,Peking University Third Hospital,Beijing 100191,China)
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2021年第4期785-788,共4页
Journal of Peking University:Health Sciences
基金
国家重点研究发展计划(2018YFC1004202)
北京市自然科学基金(7182177)
中国博士后科学基金(2017M623438)。
关键词
生殖道感染
沙眼衣原体
解脲支原体
男性
实时荧光核酸恒温扩增
Reproductive tract infections
Chlamydia trachomatis
Ureaplasma urealyticum
Male
Simultaneous amplification and testing
