摘要
将40~60日龄中华田园犬随机分为空白组、模型组、阳性药物组、复方苦芩组、针灸组和复方苦芩结合针灸组,每组30只;除空白组外,其余各组犬按照5 mL/kg(5×105 PFU/mL)灌服犬细小病毒液建立细小病毒病犬模型。复方苦芩组、针灸组和复方苦芩结合针灸组分别采用复方苦芩注射液、针灸、复方苦芩注射液结合针灸治疗。对复方苦芩注射液进行红外光谱鉴定,采用ELISA法测定犬血清中TNF-α、IL-1、IL-2及肠黏膜中IL-4、IL-5、IFN-γ水平,并制作小肠组织病理切片。结果显示,鉴定复方苦芩注射液中含有黄酮类、生物碱类、多糖类等有效物质;小肠组织病理切片显示各治疗组犬小肠组织病变和绒毛脱落情况相对模型组有好转趋势;与空白组比,模型组病犬血清与肠黏膜中各因子水平极显著升高(P<0.01);与模型组比,复方苦芩结合针灸组犬血清中IL-1、IL-2和肠黏膜中IL-4、IL-5水平极显著降低(P<0.01),而复方苦芩结合针灸组血清中TNF-α水平显著降低(P<0.05)。结果表明,复方苦芩结合针灸治疗可降低血清和肠黏膜中的炎性因子,修复病犬肠黏膜,改善炎症。
The Chinese garden dogs at 40-60 days old were randomly divided into controlgroup,model group,positive control group,compound Kuqin group,acupuncture group and compound Kuqin combined acupuncture group,30 dogs in each group.Except the control group,dogs in the other groups were fed with 5 mL/kg(5×105 PFU/mL)of canine parvovirus solution to establish a parvovirus disease dog model.The compound Kuqin group,the acupuncture group and the compound Kuqin combined acupuncture group were treated with compound Kuqin injection,acupuncture,and compound Kuqin injection combined with acupuncture,respectively.Compound Kuqin injection was identified by infrared spectroscopy,the levels of TNF-α,IL-1,IL-2 in canine serum and IL-4,IL-5,and IFN-γin intestinal mucosa were determined by ELISA,and pathological sections were made to detect the pathological changes of small intestine tissue.The results showed that compound Kuqin injection contained flavonoids,alkaloids,polysaccharides and other effective substances.Pathological sections of the small intestine showed that the pathological changes and villi loss of the small intestine tissue of dogs in each treatment group showed a trend of improvement compared with the model group.Compared with the control group,the levels of various factors in serum and intestinal mucosa of the diseased dogs in the model group increased significantly(P<0.01).Compared with the model group,the levels of IL-1 and IL-2 in serum and IL-4 and IL-5 in intestinal mucosa of the compound Kuqin combined acupuncture group extremely significantly reduced(P<0.01).The level of TNF-αin serum of the compound Kuqin combined acupuncture group significantly reduced(P<0.05).The result shows that treatment with compound Kuqin combined with acupuncture can reduce inflammatory factors in serum and intestinal mucosa,repair the intestinal mucosa,improve inflammation of diseased dogs.
作者
李珍珍
关璐
朱兆荣
刘娟
LI Zhenzhen;GUAN Lu;ZHU Zhaorong;LIU Juan(College of Veterinary Medicine,Southwest University,Rongchang,Chongqing 402460,China;Chinese Veterinary Herbal Drugs Innovation Research Laboratory,University Veterinary Science Engineering Research Center in Chongqing,Chongqing 402460,China;Medical Research Institute,South-west University,Rongchang,Chongqing 402460,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2021年第7期1384-1392,共9页
Chinese Journal of Veterinary Science
基金
科技部基础性工作专项资助项目(2013FYF110600-03)。
关键词
复方苦芩
针灸
细小病毒病
肠黏膜修复
炎性因子
compound Kuqin
acupuncture
parvovirus disease
intestinal mucosa repair
inflammatory factor
