摘要
为探讨有机氟化物和无机氟化物单一及联合暴露对斑马鱼(Danio rerio)肝脏的毒性效应,本研究将雄性斑马鱼随机分为对照组、氟化钠(NaF,无机氟)暴露组、全氟辛烷磺酸(PFOS,有机氟)暴露组、NaF+PFOS(无机氟及有机氟联合)暴露组,经15、30 d暴露试验后,检测了血液硝基蓝四氮唑(NBT)阳性细胞数及肝脏抗氧化酶和免疫相关酶活性,并观察了暴露对肝脏组织结构的影响。结果显示,暴露15 d后,NaF和PFOS单一暴露组NBT阳性细胞数以及过氧化氢酶(CAT)、碱性磷酸酶(AKP)活性均显著下降,而丙二醛(MDA)含量显著上升;暴露30 d后,NaF单一暴露组NBT阳性细胞数、超氧化物歧化酶(SOD)活性均显著下降,而MDA含量显著升高,联合暴露组酸性磷酸酶(ACP)、CAT活性较PFOS单一暴露组显著降低;而NaF+PFOS联合暴露15、30 d后均具有叠加效用。综上,NaF和PFOS单一及联合暴露均能对斑马鱼肝脏组织结构及抗氧化和免疫功能造成不同程度的毒性效应。本研究可为氟化物生态毒性效应的研究提供思路和研究基础。
To investigate the toxic effects of organic and inorganic fluoride on the liver of zebrafish(Danio rerio), healthy male zebrafish were randomly divided into control group, sodium fluoride(NaF, inorganic fluoride) group, perfluoroocatane sulfonic acid(PFOS, organic fluoride) group, and NaF+PFOS group. After exposed for 15 and 30 days, the number of NBT positive cells in blood, the activities of antioxidant enzymes and immune-related enzymes in liver, and the tissue structure of liver were determined. The results showed that after exposure for 15 days, the number of NBT positive cells, the activities of CAT and AKP were significantly reduced, while the MDA content was obviously increased in NaF group and PFOS group compared with the control group. After exposure for 30 days, NaF exposure remarkably decreased the number of NBT positive cells and the SOD activity, while increased the MDA content compared with the control exposure. The activities of ACP and CAT in NaF+PFOS group were remarkably reduced compared with the PFOS group. However, NaF and PFOS had synergistic effects after 15 and 30 days of exposure. Above all, the single and combined exposure of NaF and PFOS could cause different degrees of toxic effects on the liver structure, the antioxidant capacity and immune functions in zebrafish, which provided ideas and basis for evaluating the ecological toxic effects of fluoride.
作者
伍宜杰
孟瑞
张秀林
王国栋
陈剑杰
曹谨玲
WU Yijie;MENG Rui;ZHANG Xiulin;WANG Guodong;CHEN Jianjie;CAO Jinling(College of Veterinary Medicine,Shanxi Agricultural University,Taigu,Shanxi 030801;College of Biotechnology and Food Engineering,Anyang Institute of Technology,Anyang,Henan 455000;College of Food Science and Engineering,Shanxi Agricultural University,Taigu,Shanxi 030801)
出处
《核农学报》
CAS
CSCD
北大核心
2021年第8期1893-1899,共7页
Journal of Nuclear Agricultural Sciences
基金
山西省回国留学人员科研资助项目(2020-061)
山西省重点研发计划项目(201903D221009)
国家自然科学基金(31440087、31502141)
中国博士后科学基金项目(2012M520601、2013T60267)。
关键词
斑马鱼
氟化钠
全氟辛烷磺酸
抗氧化酶
免疫相关酶
zebrafish
sodium fluoride
perfluorooctane sulfonic acid
antioxidant enzymes
immune-related enzymes
