摘要
为了评估一株解淀粉芽孢杆菌(Bacillus amyloliquefaciens,菌株编号YNAU 6172)对畜禽肠道主要有害菌(大肠杆菌K88、大肠杆菌K99、猪霍乱沙门氏菌、鸡白痢沙门氏菌)及肠道有益菌(罗伊氏乳酸杆菌、嗜热双歧杆菌)的抑制作用,采用牛津杯体外抑菌试验法,以抑菌圈直径值判定解淀粉芽孢杆菌对以上6个指示菌的抑制作用。结果显示:解淀粉芽孢杆菌对4个肠道有害菌的抑制作用均判定为不敏感,对肠道有益菌嗜热双歧杆菌判定也为不敏感,但对肠道有益菌罗伊氏乳酸杆菌的抑制作用为敏感。说明解淀粉芽孢杆菌作为饲用微生态制剂开发需进一步筛选和验证。
In order to evaluate the inhibitory effect of a Bacillus amyloliquefaciens YNAU 6172 strain on intestinal harmful bacteria(Escherichia coli K88,Escherichia coli K99,Salmonella choleraesuis,and Salmonella pullorum)and intestinal beneficial bacteria(Lactobacillus reuteri and Bifidobacterium thermophilum)of feeding animal,the diameter of inhibition zone were determined by the Oxford cup method to indicate the antibacterial properties of Bacillus amyloliquefaciens on the above six indicator bacteria.The results showed that the strain Bacillus amyloliquefaciens had"insensitive"inhibitory effects on four indicator bacteria of intestinal harmful bacteria and intestinal beneficial bacteria Bifidobacterium thermophilum,and"Sensitive"on intestinal beneficial bacteria Lactobacillus reuteri.These results suggested that the utilization of Bacillus amyloliquefaciens as a probiotics feed additive need further isolation,identification and verification.
作者
王政露
肖明霞
王乙茹
柳成东
赵平
钱锦花
杨保和
屈娥
程志斌
WANG Zhenglu;XIAO Mingxia;WANG Yiru;LIU Chengdong;ZHAO Ping;QIAN Jinhua;YANG Baohe;QU E;CHENG Zhibin(Yunnan Agricultural University,Kunming 650201,China;Dazhou Vocational and Technical College,Dazhou 635001,Sichuan China;Yuxi Agriculture Vocation-Tecanical College,Yuxi 653100,Yunnan China;Yunnan Kuaidaduo Animal Husbandry Technology Limited Company,Yuxi 653100,Yunnan China)
出处
《饲料博览》
2021年第8期1-5,9,共6页
Feed Review
基金
云南农业大学博士科研启动基金(A2032002455)
云南省创新团队项目(2017HC030)。
关键词
解淀粉芽孢杆菌
抑菌性能
牛津杯法
肠道有害菌
肠道有益菌
Bacillus amyloliquefaciens
antibacterial property
oxford cup method
intestinal harmful bacteria
intestinal beneficial bacteria
