摘要
利用在西瓜全基因组开发的446对SSR引物,筛选出一套覆盖西瓜11条染色体,分布均匀,多态性较高的110对引物,在此基础上分析81份西瓜种质资源遗传多样性。结果表明,检测到每对引物等位基因数变化为2~8个,平均3.4个,PIC值平均为0.4。聚类分析结果表明,将供试材料分为两大类群;通过全基因组关联分析检测到与果皮厚度显著相关SSR标记2个,分别位于4、9号染色体,与中心果肉可溶性固形物含量显著相关SSR标记2个,分别位于6、11号染色体,与种子厚度显著相关SSR标记1个,位于4号染色体。
Based on 446 SSR primers developed on the whole watermelon genome,a set of 110 primers covering 11 chromosomes of watermelons with uniform distribution and high polymorphism were screened out.Based on these primers,the genetic diversities of 81 watermelon germplasm resources were analyzed.The results showed that the number of alleles per pair of primers varied from 2 to 8,with an average of 3.4,and the average PIC value was 0.4.The results of cluster analysis showed that the tested materials were divided into two groups.Detected through a genome-wide association analysis and the thickness of the skin were two significant correlation SSR markers,respectively located on chromosome 4,9 and center the fruit soluble solids content were two significant correlation SSR markers,located on chromosome 6 and 11,respectively,with one was significantly associated with the seed thickness of SSR markers,located on chromosome 4.
作者
栾非时
闫令文
刘树森
刘钊
高鹏
宋正峰
朱子成
刘宏宇
LUAN Feishi;YAN Lingwen;LIU Shusen;LIU Zhao;GAO Peng;SONG Zhengfeng;ZHU Zicheng;LIU Hongyu(Key Laboratory of Biology and Genetic Improvement Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs,Harbin 150030,China;School of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,China;Sanmu Seeds Limited Company in Shouguang City of Shandong Province,Shouguang Shandong 262704,China;School of Agriculture and Environment,Weifang University of Science and Technology,Shouguang Shandong 262799,China)
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2021年第7期19-32,共14页
Journal of Northeast Agricultural University
基金
国家重点研发计划子课题(2018YFD0100703)
泰山产业领军人才工程(高效生态农业创新类)项目。
关键词
西瓜
种质资源
SSR标记
背景选择
watermelon
germplasm resource
SSR marker
background selection
