摘要
天山雪莲是我国传统中药材,对于风寒湿痹痛、类风湿性关节炎及小腹冷痛、月经不调有良好的疗效。然而,该药材生境特异,自然繁殖率低,生长慢,长期掠夺性采挖使其野生资源濒临灭绝。应用植物细胞培养技术,通过培养愈伤组织、悬浮细胞和毛状根可获得天山雪莲细胞,是天山雪莲药材的一个新来源。培养技术方面,作者重点综述了反应器类型、培养体系、生物合成前体、诱导子类型以及光谱特性等对雪莲细胞悬浮培养的影响。天山雪莲细胞的化学成分主要有苯丙素类、黄酮类、木脂素类和甾体类等,其中以苯丙素类物质种类最为丰富。药理方面,天山雪莲细胞具有抗炎镇痛、活血化瘀、免疫调节、增加骨密度、降血脂、抗缺氧、抗运动性疲劳、抗辐射、抗肥胖、抗氧化以及治疗再生障碍性贫血等多方面的作用。该研究对天山雪莲细胞的培养技术、化学成分和药理作用进行了综述,为其深入研究和开发利用提供依据。
Saussurea involucrata,a traditional Chinese medicinal material,is effective in the treatment of rheumatoid arthritis with cold-dampness blockage syndrome,cold pain in lower abdomen,and menstrual irregularities.However,due to the specific habitat,low natural reproduction rate,slow growth,and overexploitation,it is at the high risk of extinction.S.involucrata cells can be obtained through callus culture,suspension culture,and hairy root culture.This study highlighted the influences of reactor type,culture system,precursor,elicitor type,and light wavelength on the suspension culture of S.involucrate cells.The chemical components of S.involucrata cells mainly include phenylpropanoids,flavonoids,lignans,and steroids,among which phenylpropanoids are the most abundant.S.involucrata cells have multiple pharmacological activities of anti-inflammation,analgesia,activating blood and resolving stasis,immunoregulation,increasing bone density,lowering blood lipids,anti-hypoxia,anti-exercise fatigue,anti-radiation,anti-obesity,and anti-oxidation.Moreover,it has the potential of treating aplastic anemia.This study reviews the cell culture technologies,chemical components,and pharmacological activities of S.involucrata cells,laying a basis for the further research,development,and utilization.
作者
范文霞
杨伟鹏
刘汉石
FAN Wen-xia;YANG Wei-peng;LIU Han-shi(Dalian Practical Biotechnology Co.,Ltd.,Dalian 116001,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第14期3522-3528,共7页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2018YFC1706100)。
关键词
天山雪莲细胞
雪莲培养物
天山雪莲
植物细胞培养技术
化学成分
药理作用
研究进展
Saussurea involucrata cells
Saussurea involucrata culture
Saussurea involucrata
plant cell culture technology
chemical component
pharmacological activity
research progress
