摘要
目的建立HPLC法同时测定赶黄草中乔松素-7-O-β-D-葡萄糖苷、pinocembrin-7-O-[4″,6″-(S)-hexahydroxydiphenoyl]-β-D-glucose、thonningianin A、pinocembrin-7-O-[3″-O-galloyl-4″,6″-(S)-hexahydroxydiphenoyl]-β-Dglucose的含量。方法赶黄草80%甲醇提取物的分析采用Agilent ZORBAX SB-C18柱(4.6 mm×250 mm,5μm)流动相乙腈-0.5%甲酸水,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长280 nm。结果4种成分在各自范围内线性关系良好(r≥0.9999),平均加样回收率100.90%~102.04%,RSD 0.82%~2.54%。结论该方法准确稳定,重复性好,可用于赶黄草的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of pinocembrin-7-O-β-D-glucoside,pinocembrin-7-O-[4″,6″-(S)-hexahydroxydiphenoyl]-β-D-glucose(pinocembrin-7-O-[4″,6″-(S)-hexahydroxydiphenoyl]-β-D-glucose),thonningianin A and pinocembrin-7-O-[3″-O-galloyl-4″,6″-(S)-hexahydroxydiphenoyl]-β-D-glucose(pinocembrin-7-O-[3″-O-galloyl-4″,6″-(S)-hexahydroxydiphenoyl]-β-D-glucose)in Penthorum chinense Pursh.METHODS The analysis of 80%methanol extract of P.chinense was performed on a 30℃thermostatic Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm),with the mobile phase comprising of acetonitrile-0.5%formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS Four constituents showed good linear relationships within their own ranges(r≥0.9999),whose average recoveries were 100.90%-102.04%with the RSDs of 0.82%-2.54%,respectively.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of P.chinense.
作者
史鹏杰
程嘉希
黄豆豆
董志颖
孙连娜
SHI Peng-jie;CHENG Jia-xi;HUANG Dou-dou;DONG Zhi-ying;SUN Lian-na(School of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《中成药》
CAS
CSCD
北大核心
2021年第9期2408-2411,共4页
Chinese Traditional Patent Medicine
基金
国家重点研发计划资助(2019YFC1711000)
上海市自然科学基金项目(13401900106)。
