摘要
青黛的药用历史悠久,其加工炮制过程具有特殊性及复杂性。明代以前,药用青黛取打靛形成的顶层泡沫状物的干燥品“靛花”,现代生产工艺将打靛形成沉淀物“粗靛”除杂干燥后制得青黛成品,古今青黛饮片形式已经发生了重大的转变。因此,该文采用现代分析技术对粗靛与靛花进行质量对比研究。首先基于UPLC-Q-TOF-MS进行化学成分分析,HS-SPME/GC-MS/MS方法分析气味化学成分,测定粗靛和靛花中靛蓝、靛玉红、总灰分与水溶性浸出物的含量,无机成分组成,并且观察其微观形态及表面元素组成。最后通过脂多糖和2,4-二硝基苯酚致大鼠发热模型对靛花和粗靛的解热活性进行对比,发现靛花解热作用起效更快且药效更持久,而粗靛的解热作用不明显。该研究对青黛加工炮制工艺研究具有重要意义,为青黛的质量标准制定与现代炮制装备研制提供了参考。
Indigo Naturalis has a long history of medicinal use with particularity and complexity in its processing.Before the Ming dynasty,Indigo Naturalis was extracted from the top layer of zymotic fluid,called"purified Indigo Naturalis".In modern processing,the precipitate"crude Indigo Naturalis"is dried to produce Indigo Naturalis after impurity removal.The form of Indigo Naturalis slices has undergone significant changes in ancient and modern times.In view of this,the quality comparison between crude Indigo Naturalis and purified Indigo Naturalis was conducted in this study with modern analytical techniques.Firstly,chemical composition was analyzed with UPLC-Q-TOF-MS,and the chemical composition of scent with HS-SPME/GC-MS/MS.The content of indigo,indirubin,total ash,and water-soluble extract was determined as well as the inorganic composition in crude Indigo Naturalis and purified Indigo Naturalis.Then,their microscopic morphology was observed and the surface element composition was investigated.Finally,the antipyretic activities of crude Indigo Naturalis and purified Indigo Naturalis were compared in the fever rat model induced by lipopolysaccharide and 2,4-dinitrophenol.The results demonstrated that the purified Indigo Naturalis had a faster and more lasting antipyretic effect,while the crude Indigo Naturalis had almost no antipyretic effect.This study is of great significance to the research on processing technology of Indigo Naturalis and provides reference for the formulation of its quality standards,production specifications and calibration procedures.
作者
马乐乐
许润春
张定堃
陈利明
吴意
黄浩洲
王芳
林俊芝
韩丽
杨明
MA Le-le;XU Run-chun;ZHANG Ding-kun;CHEN Li-ming;WU Yi;HUANG Hao-zhou;WANG Fang;LIN Jun-zhi;HAN Li;YANG Ming(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Sichuan Lianchengxunkang Medical&Pharmaceutical Co.,Ltd.,Yaan 625000,China;Key Laboratory of Modern Chinese Medicine Preparation of Ministry of Education,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;Traditional Chinese Medicine Regulating Metabolic Diseases Key Laboratory of Sichuan Province,Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610072,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第13期3188-3197,共10页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2018YFC1707205)
国家自然科学基金面上项目(81773918)
成都中医药大学西南特色中药资源重点实验室开放研究基金项目(2020XSGG020)
江西中医药大学创新药物与高效节能降耗制药设备国家重点实验室开放基金项目(GZSYS202003)。
关键词
青黛
粗靛
靛花
炮制
质量对比
解热
Indigo Naturalis
crude Indigo Naturalis
purified Indigo Naturalis
processing technology
quality comparison
antipyretic
