摘要
新型冠状病毒的全球大流行,给世界人民的生命健康带来了巨大威胁。目前,针对新型冠状病毒的预防和治疗措施仍然非常有限。核酸检测对于实现快速确诊感染病例、排除疑似病例、促进疫情的防控与临床救治至关重要。本文通过综述RT-PCR技术在新型冠状病毒核酸检测中的应用及面临的挑战,总结经过本实验室长时间积累的实践经验,如增加仪器设备、优化实验流程、加强信息化建设、改善工作条件等措施以提高核酸检测的能力和效率,为实验室新型冠状病毒核酸检测提供参考依据。
The global pandemic of novel coronavirus has brought great threat to the life and health of people all over the world.Currently,the prevention and treatment measures for novel coronavirus remain very limited.Nucleic acid testing is crucial for the rapid diagnosis of infected cases,the exclusion of suspected cases,and the promotion of epidemic prevention and control and clinical treatment.In this paper,the application and challenges of RT-PCR technology in novel coronavirus nucleic acid detection are reviewed,and the practical experience accumulated in our laboratory for a long time is summarized,such as increasing instruments and equipment,optimizing experimental process,strengthening information construction,and improving working conditions,to improve the ability and efficiency of nucleic acid testing,and provide a reference for nucleic acid detection of novel coronavirus in the laboratory.
作者
宋江勤
陈玫君
曹伟伟
康娜
吕文静
周君阳
Song Jiangqin;Chen Meijun;Cao Weiwei;Kang Na;Lyu Wenjing;Zhou Junyang(The First Poeple's Hospital of Tianmen City,Tianmen Hubei 431700,China)
出处
《中华临床实验室管理电子杂志》
2021年第3期129-132,149,共5页
Chinese Journal of Clinical Laboratory Management(Electronic Edition）
