摘要
【目的】为了探究添加肉桂醛对Na_(2)SO_(4)胁迫下烟草幼苗生长的影响机制。【方法】以烤烟K326为材料,在烟草幼苗期用不同浓度的Na_(2)SO_(4)和肉桂醛进行处理,结合烟草幼苗根系发育状况、活性氧含量、抗氧化酶活性及相关基因表达量等指标进行分析。【结果】Na_(2)SO_(4)浓度越高,对烟草生长的抑制作用越明显,烟苗根系发育越缓慢,H_(2)O_(2)积累越多;75 mmol/L Na_(2)SO_(4)胁迫下,0~25μmol/L肉桂醛可以促进烟草幼苗根系生长,降低H_(2)O_(2)和MDA含量,诱导降低SOD和POD的酶活,提高CAT酶活,从而提高幼苗的抗氧化能力,增强烟苗的抗盐能力。【结论】在烟草幼苗阶段,20μmol/L的肉桂醛可以有效提高烟草幼苗对75 mmol/L Na_(2)SO_(4)的抵抗能力,主要通过促进根系发育,调节活性氧含量,诱导抗氧化物质活性变化,缓解盐胁迫的抑制作用。
[Objective]This study aims to investigate the effect of cinnamaldehyde additive on the growth of tobacco seedlings under Na_(2)SO_(4) stress.[Methods]Flue-cured tobacco K326 was treated with different concentrations of Na_(2)SO_(4) and cinnamaldehyde at the seedling stage.The physiological indexes such as root length,ROS content and antioxidant enzyme content of tobacco seedlings were analyzed.[Results]The higher the concentration of Na_(2)SO_(4),the more obvious the inhibition effect of cinnamaldehyde additive on tobacco growth,the slower tobacco root development,and the higher H_(2)O_(2) content.Exogenous cinnamaldehyde can promote the growth of tobacco seedling roots,reduce the content of H_(2)O_(2) and MDA,improve the antioxidant capacity of tobacco seedlings,and enhance the salt resistance of tobacco seedlings.[Conclusion]At the stage of tobacco seedling,20μmol/L cinnamaldehyde could enhance the resistance of tobacco seedlings against 75 mmol/L Na_(2)SO_(4).The inhibition effect of salt stress was alleviated mainly by promoting root development,regulating the content of reactive oxygen species,inducing the change of antioxidant activity.
作者
刘晓涵
李雪利
王悦霖
宋佳倩
徐亮
叶协锋
吴东
王静
LIU Xiaohan;LI Xueli;WANG Yuelin;SONG Jiaqian;XU Liang;YE Xiefeng;WU Dong;WANG Jing(College of Tobacco Science,Henan Agricultural University,National Tobacco Cultivation and Physiology and Biochemistry Research Center,Key Laboratory for Tobacco Cultivation of Tobacco Industry,Zhengzhou 450002,China;Guangdong Shaoguan Tobacco Recuring Co.,LTD.,Shaoguan 512000,China;Staff development institute China National Tobacco Corporation,Zhengzhou 450002,China;China Tobacco Hubei Industry LLC,Wuhan 430000,China)
出处
《中国烟草学报》
CAS
CSCD
北大核心
2021年第4期59-65,共7页
Acta Tabacaria Sinica
基金
国家自然基金(No.41907076)
烟草行业烟草栽培重点实验室项目(No.30800665)。
关键词
烟草
硫酸钠
肉桂醛
盐胁迫
tobacco
sodium sulfate
cinnamaldehyde
salt stress
