摘要
对蛋清料液进行前处理:采用磷酸盐缓冲液稀释10倍,在24MPa压力下均质;对超滤工艺进行了研究,采用截留相对分子质量为30000的聚醚砜(PES)膜进行超滤;溶菌酶制品活力为14610U/mg.
Lysozyme was isolated from egg white by using ultrafiltration(UF) technique. The preparation procedures were as followings: egg white was diluted with phosphate buffer by 10 times, humogenated at 24 MPa; the ultrafiltration process was studied by using PES membrane with 30 000 MWCO. The enzyme activity of final preparation was 14 610 U/mg.
出处
《无锡轻工大学学报(食品与生物技术)》
CSCD
北大核心
2002年第6期607-612,共6页
Journal of Wuxi University of Light Industry
关键词
超滤法
提取
蛋清
溶菌酶
膜通量
酶活
Lysozyme
Ultrafiltration
Egg White
Membrane Permeate Flux