摘要
以一株大肥蘑菇(Agaricus bitorquis)为试材,通过单因素试验筛选该菌株的最适碳源、氮源、无机盐、温度和pH,在这个基础上,再通过正交试验,寻找该菌株的最适固体培养条件组合。单因素试验结果表明该株大肥蘑菇菌丝的最适生长碳源为麦芽糖,氮源为麸皮,无机盐为硫酸钙,温度为20℃,pH为6.5。正交试验表明该株大肥蘑菇的最适生长条件组合是麦芽糖、麸皮、20℃和pH 6.5。
Take an Agaricus bitorquis as the test material,the optimal carbon source,nitrogen source,inorganic salt,pH and temperature of the strain were screened by single factor test.On this basis,the optimal combination of solid culture conditions of the strain was found by orthogonal test.Single factor analysis showed that the most suitable carbon source for the strain was maltose;nitrogen source was bran;inorganic salt was calcium sulfate;temperature was 20℃;and pH was 6.5.Orthogonal test showed that the optimal growth condition combination of this strain’s hyphae was maltose,bran,20℃and pH 6.5.
作者
刘明广
张娜娜
郭凤军
LIU Mingguang;ZHANG Nana;GUO Fengjun(Department of Biochemical Engineering, Fuyang Vocational and Technical College, Fuyang, Anhui 236031,China;Funan County Zhongben Agricultural Technology Company Limited, Funan, Anhui 236300, China)
出处
《合肥师范学院学报》
2021年第3期10-13,共4页
Journal of Hefei Normal University
基金
安徽省科技厅重点研究与开发计划项目“大肥蘑菇的示范栽培及菌渣有机肥在蔬菜种植上的关键技术研究”(202004f06020016)。
关键词
大肥蘑菇
培养条件
单因素
正交试验
Agaricus bitorquis
culture condition
single factor
orthogonal test
