摘要
通过生物信息学分析,将从苹果全基因组中鉴定出的11个组氨酸激酶蛋白基因(MdAHKs)依次命名为MdAHK1~MdAHK11,依据是这些基因在染色体上的位置;此外,对MdAHK1~MdAHK11的理化特性、系统进化关系、蛋白基序及启动子元件进行了分析.MdAHK编码的蛋白大小为901~1230 aa;等电点介于4.95和8.56之间;对蛋白保守基序的分析发现,MdAHKs家族成员中有4~10个保守基序,根据系统进化分析将MdAHKs家族11个成员分为了A1,A2和A3共3个亚家族.此外,利用RT-qPCR技术检测了试验材料苹果砧木“M9-T337”中MdAHKs在不同组织以及不定根发育不同时期的表达量;结果发现MdAHKs家族中MdAHK1,MdAHK4,MdAHK5和MdAHK9在根、茎中高表达,同时响应6-BA处理表达量显著下调,Lovastatin(细胞分裂素抑制剂)处理表达量呈上调趋势.结合生物信息学分析和荧光定量结果推测MdAHK4,MdAHK5,MdAHK9可能在介导苹果砧木不定根的发育过程中发挥重要作用.
In this study,a total of 11 histidine kinase protein(AHK)were identified from the apple genome by bioinformatics.According to the position of the gene in the chromosome,MdAHK genes were named MdAHK1-MdAHK11.Additionally,we further systematically analyzed its physical and chemical characteristics,evolutionary relationships,Protein motif and promoter elements.MdAHK proteins contained from 901 to 1230 amino acids and the isoelectric points is from 4.95 to 8.56.Conservative base sequence analysis showed that 4-10 conserved motifs existed in the MdAHK proteins.These genes could be classified into three subgroups(A1,A2 and A3)according to its homology by phylogenetic analysis.Additionally,‘M9-T337’apple rootstock were as material,fluorescence quantitative PCR confirmed the changes of MdAHK family genes expression in different tissues and different stages during adventitious root formation.The results showed that MdAHK1,MdAHK4,MdAHK5 and MdAHK9 of the MdAHKs family were highly expressed in roots and stems,and the expression level was significantly down-regulated in response to 6-BA treatment,and was significantly up-regulated under lovastatin(cytokinin inhibitor)treatment.The results of bioinformatics analysis and fluorescence quantitative analysis showed that MdAHK4,MdAHK5 and MdAHK9 might be involved in the regulation of adventitious root development of apple rootstock.
作者
鲍俊华
李珂
王程程
张东
BAO Junhua;LI Ke;WANG Chengcheng;ZHANG Dong(College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China)
出处
《西南师范大学学报（自然科学版）》
CAS
2021年第9期65-74,共10页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家重点研发计划项目(2019YFD1000803)
陕西省科技统筹重点项目(2017NY0055)
国家现代农业产业技术体系建设专项资金项目(CARS-27)
“仲英青年学者”项目.
