摘要
为验证一种浮游植物快速浓缩固定方法——滤膜浓缩法的可靠性,采用“鲁戈氏液固定沉降法”和“滤膜浓缩法”同时鉴定了不同浮游植物浓度梯度水样,并进行了对比分析,结果表明:鲁戈氏液固定沉降法与滤膜浓缩法整体上均鉴定出7门,32属,但不同梯度上滤膜浓缩法鉴定出的浮游植物种类较多.两种方法门水平上除裸藻门(P<0.05)外丰度均无明显差异(P>0.05),属水平上相对丰度99%以内的浮游植物均无明显差异(P>0.05),即无论是门还是属水平上,滤膜浓缩法对浮游植物丰度的鉴定是可行的.采用两种方法得到的不同梯度下藻密度拟合方程斜率差异较小(P>0.05)且相关性显著(R^(2)=0.958,P<0.01),但滤膜浓缩法鉴定出的浮游植物细胞密度更大.整体来看,滤膜浓缩法得到的藻类鉴定结果与鲁戈氏液固定沉降法基本一致.
In order to verify the reliability of a membrane concentration method to rapidly concentrate and fixe phytoplankton in the field,the"Lugol's solution fixed sedimentation method(LSM)"and"membrane concentration method(MCM)"were comparatively used here to authenticate different phytoplankton samples.The results show that:(1)7 phyla and 32 genera were both identified by the two method,but using the MCM could authenticate more phytoplankton species than the LSM method;(2)There was no significant difference not only in phytoplankton abundance between the two methods except Euglenophyta(P<0.05)at the phylum level(P>0.05),but also in phytoplankton relative abundance within 99% at the genus level(P>0.05).Which meant that,at the phylum and genus levels,the MCM was feasible to identify the phytoplankton abundance;(3)There was no significant difference in the slope of fitting equation of different algae biomass gradients(P>0.05),and they showed a high correlation coefficient(R^(2)=0.958,P<0.01),however,algal biomass of the MCM seemed to be a little larger.On a conclusion,the results of the MCM was consistent with the LSM,and the MCM had more advantages in less environmental condition requirement,easier for field monitoring,and save time.
作者
李亚莉
杨正健
田盼
刘毅
王从锋
纪道斌
刘德富
LI Ya-li;YANG Zheng-jian;TIAN Pan;LIU Yi;WANG Cong-feng;JI Dao-bin;LIU De-fu(Hubei Field Observation and Scientific Research Stations for Water Ecosystem in Three Gorges Reservoir,China Three Gorges University,Yichang 443002,China;Hubei Key Laboratory of Ecological Restoration of River-lakesand Algal Utilization,Hubei University of Technology,Wuhan 430068,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2021年第9期4300-4309,共10页
China Environmental Science
基金
国家自然科学基金项目(51879099、51779128和51909135)。
关键词
鲁戈氏液固定沉降法
滤膜浓缩法
物种种类
物种丰度
线性拟合
Lugol's solution fixed sedimentation method
membrane concentration method
phytoplankton species
species abundance
linear fitting
