摘要
以自然老化甜菜种子法饲一号为试验材料,研究不同引发处理对老化甜菜种子活力和幼苗生理特性的影响。结果表明,与对照相比各处理均不同程度改善了老化种子活力及生理相关指标,其中12 h纯水浸种、1.0%壳聚糖、250 mg·L^(-1)GA_(3)、0.6%H_(2)O_(2)、25%PEG-6000、1.5%CaCl_(2)及100 mg·L^(-1)AsA分别为各引发剂最佳处理。经1.0%壳聚糖和250 mg·L^(-1)GA_(3)引发后的种子萌发相关指标显著高于其他处理,淀粉酶活力及可溶性蛋白含量显著升高,幼苗活性氧含量与脂膜过氧化作用显著降低(p<0.05)。说明引发不仅可以提高老化甜菜种子活力和萌发相关酶活性,还可以减轻老化种子细胞膜受伤害程度,进而有利于幼苗后续生长发育。
In this study,the effects of different priming treatments on seed vigor and seedling physiological characteristics of aging beet were studied using natural aging beet variety Fasi 1 as experimental material.The results showed that all treatments improved the vigor and physiological indexes of aging seeds to different degrees compared with the control.Among them,the pure water soaking for 12 h,1.0%chitosan,250 mg·L^(-1)GA_(3),0.6%H_(2)O_(2),25%PEG-6000,1.5%CaCl_(2)and 100 mg·L^(-1)AsA were the best treatments for each initiating agent.The seed germination-related indexes induced by 1.0%chitosan and 250 mg·L^(-1)GA_(3)were significantly higher than those of other treatments,amylase activity and soluble protein content were significantly increased,and reactive oxygen species content and lipid membrane peroxidation of seedlings were significantly decreased(p<0.05).These results indicated that priming could not only improve the vigor and germination-related enzyme activities of aging beet seeds,but also reduce the damage degree of cell membrane of aging seeds,which was beneficial to the subsequent growth and development of seedlings.
作者
荆文旭
魏磊
万雪
石鑫鑫
郭明
邢旭明
史树德
JING Wenxu;WEI Lei;WAN Xue;SHI Xinxin;GUO Ming;XING Xuming;SHI Shude(College of Agronomy,Inner Mongolia Agricultural University,Hohhot 010019,China)
出处
《种子》
北大核心
2021年第8期116-121,126,共7页
Seed
基金
国家现代农业产业技术体系(CARS-170702)。
关键词
甜菜
自然老化种子
引发
幼苗生理特性
淀粉酶活力
sugar beet
natural aging seed
priming
seedling physiological characters
amylase activity
