摘要
本研究为揭示软枣猕猴桃中无色花色素双加氧酶(leucoanthocyanidin dioxygenase, LDOX)基因结构及其功能。运用生物信息学方法对软枣猕猴桃LDOX核苷酸及氨基酸序列进行深入分析,构建LDOX基因的蛋白互作网络,并进行GO及KEGG功能注释分析。结果显示:软枣猕猴桃与野茶树、以南高丛蓝莓、蓝莓、金银花及胡萝卜亲缘关系较近,含有10个保守基序。顺式调控元件主要参与ABA信号途径、响应干旱应答,厌氧诱导、赤霉素、生长素响应因子、Me JA反应、低温响应、光刺激应答响应元件。LDOX理化性质及结构分析表明,其为亲水性蛋白,未发现信号肽及跨膜结构区域,主要包括Ser磷酸化位点(13个)、Thr磷酸化位点(12个)及Tyr磷酸化位点(6个)。含有两个O-糖基化位点(Thr4和Thr323),并且在107~109残基位置存在Asn107N-糖基化位点。48~347 bp处含有2-酮戊二酸-Fe2+-双加氧酶家族(20G-FeⅡOxy)保守结构域,二级结构主要以无规则卷曲(43.10%)为主。LDOX和F3H、UFGT、FLS及PAL等蛋白之间存在一定互作关系,参与类黄酮生物合成及代谢过程,主要在植物型液泡膜中发挥作用,并且具有调控催化活性及氧化还原活性分子功能。LDOX基因高度保守,在类黄体酮通路中发挥重要的调控作用,为进一步深入探讨软枣猕猴桃LDOX基因功能机制提供了理论依据。
The purpose of this study is to reveal the structure and function of the leucoanthocyanidin dioxygenase(LDOX) gene in Actinidia arguta. Bioinformatics methods were used to analyze the LDOX nucleotide and amino acid sequences of Actinidia arguta, construct the protein interaction network of LDOX gene, and conduct GO and KEGG functional annotation analysis. The results showed that there were 10 conserved motifs in Actinidia arguta,which was closely related to Vaccinium corymbosum, Lonicera japonica and Daucus carota. Cis-regulated elements were mainly involved in ABA signaling pathways, responding to drought response, anaerobic induction, gibberellic acid, auxin response factor, MeJA response, low temperature response, and photostimulus response. Physical and chemical properties and structural analysis of LDOX showed that it was hydrophilic protein, and no signal peptide and transmembrane structural regions were found, mainly including Ser phosphorylation sites(13), Thr phosphorylation sites(12) and Tyr phosphorylation sites(6). There werw two O-glycosylation sites(Thr4 and Thr323) and Asn107 of N-glycosylation sites at residues ranging from 107 to 109. There was a domain structure conservative in 48~347 bp was 20 G-FeⅡ Oxy. The secondary structure main random coil(43.10%). There was a certain interaction between LDOX and proteins such as F3 H, UFGT, FLS and PAL, which involved in flavonoid biosynthesis and metabolism process that plays an important role in plant-type vacuole membrane, and regulates the function of catalytic activity and redox active molecule. LDOX gene was highly conserved and plays an important regulatory role in progesterone pathway, which provides a theoretical basis for further research on the functional mechanism of LDOX gene in Actinidia arguta.
作者
刘振盼
卢立媛
孙阳
张悦
李冬生
尤文忠
Liu Zhenpan;Lu Liyuan;Sun Yang;Zhang Yue;Li Dongsheng;You Wenzhong(Liaoning Economic Forest Research Institute,Dalian,116031)
出处
《分子植物育种》
CAS
北大核心
2021年第16期5286-5296,共11页
Molecular Plant Breeding
基金
辽宁省“兴辽英才计划”项目(NO.XLYC1902015)
辽宁省中央引导地方科技发展专项(NO.2019JH6/102-00005)共同资助。
关键词
软枣猕猴桃
LDOX
结构功能
蛋白互作
Actinidia arguta
LDOX
Structure function
Protein interaction
