摘要
目的:探讨乳酸杆菌对新生鼠和成年鼠辅助性T细胞(Th)1/Th2平衡及肺树突状细胞(DC)表型的影响。方法:新生鼠从出生后连续7 d每天经口给予109 CFU/mL浓度乳酸杆菌10μL,成年鼠选取满6周的小鼠连续7 d每天经口给予109 CFU/mL浓度乳酸杆菌50μL,实验第8天进行盲肠内容物培养以确定乳酸杆菌肠道定植情况。干预3周后,酶联免疫吸附试验(ELISA)检测血清干扰素(IFN)-γ、白细胞介素(IL)-4和IL-10水平,流式细胞仪检测肺树突状细胞亚型和成熟度。结果:(1)乳酸杆菌干预的新生鼠乳酸杆菌数量较对照组增加,而肠球菌和肠杆菌数量降低(P<0.01),乳酸杆菌干预的成年鼠肠道乳酸杆菌数量较对照组增加(P<0.01),但肠道肠球菌和肠杆菌数量没有变化。(2)乳酸杆菌干预的新生鼠IFN-γ和IL-10水平较对照组高(P<0.05),IL-4水平两组比较差异无统计学意义。乳酸杆菌干预的成年鼠IFN-γ、IL-4和IL-10水平与对照组比较差异无统计学意义。(3)乳酸杆菌干预的新生鼠肺CD11c^(+)CD8α^(-)水平为92.34%±0.94%,较对照组降低(P<0.05),干预组CD11c^(+)CD8α^(+)水平较对照组增加(P<0.05)。经过乳酸杆菌干预后,新生鼠肺部14.03%±1.14%的DC表达MHCⅡ类分子,较对照组明显增加。成年鼠乳酸杆菌干预组CD11c^(+)CD8α^(-)、CD11c^(+)CD8α^(+)和CD11c^(+)MHCⅡ+水平与对照组比较差异无统计学意义(P>0.05)。结论:乳酸杆菌干预可以促进新生鼠Th1/Th2平衡向Th1方向偏移,而对成年鼠没有影响。
Objective:To probe into the effects of Lactobacillus on helper T cell 1(Th1)/helper T cell 2(Th2)balance and lung dendritic cell(DC)phenotype of neonatal mice and adult mice.Methods:Neonatal mice were orally administered 10μL Lactobacillus at the concentration of 10^(9) CFU/mL every day for consecutive 7 d after birth.Adult mice at the age of 6 weeks were orally administered with 50μL Lactobacillus at the concentration of 109 CFU/mL every day for consecutive 7 d.Culture of cecum contents was performed on the 8th day of the experiment to determine the intestinal colonization of Lactobacillus.After 3 weeks of intervention,serum interferon(IFN)-γ,interleukin(IL)-4 and IL-10 were measured by enzyme-linked immunosorbent assay(ELISA),and lung dendritic cell subtype and maturation were detected by flow cytometry.Results:(1)Compared with the control group,the number of Lactobacillus in neonatal mice with Lactobacillus intervention increased,while the number of Enterococcus and Enterobacter decreased(P<0.01),the number of Lactobacillus in adult mice with Lactobacillus intervention were higher than those in the control group(P<0.01),yet the number of Enterococcus and Enterobacter in adult mice did not changed.(2)Compared with the control group,the levels of IFN-γand IL-10 of neonatal mice with Lactobacillus intervention increased(P<0.05),there was no significant difference in the level of IL-4 between two groups.The difference was not statistically significant in the levels of IFN-γ,IL-4 and IL-10 between the adult mice with Lactobacillus intervention and the control group.(3)The level of CD11 c^(+)CD8α^(-)of neonatal mice with Lactobacillus intervention was 92.34%±0.94%,lower than that of the control group(P<0.05),and the level of CD11 c^(+)CD8α^(+)in the intervention group was higher than that in the control group(P<0.05).After intervention by Lactobacillus,14.03%±1.14%of DC in the lung of neonatal mice expressed MHCⅡmolecules,significantly higher than that in the control group.There was no significant difference in levels of CD11 c^(+)CD8α^(-),CD11 c^(+)CD8α^(+)and CD11 c^(+)MHCⅡ+between the adult mice with Lactobacillus intervention group and the control group(P>0.05).Conclusion:Lactobacillus intervention can promote the shift in Th1/Th2 balance toward Th1 in neonatal mice,yet has no effect on adult mice.
作者
谢娜
刘崇海
皮光环
雷勋明
蔡艳
刘昊昊
李小林
Xie Na;Liu Chonghai;Pi Guanghuan;Lei Xunming;Cai Yan;Liu Haohao;Li Xiaolin(Sichuan Provincial Maternity and Child Health Hospital,Sichuan Chengdu 610031,China;North Sichuan Medical College,Sichuan Nanchong 637000,China)
出处
《儿科药学杂志》
CAS
2021年第9期1-4,共4页
Journal of Pediatric Pharmacy
基金
四川省教育厅重点项目,编号16ZA0227
川北医学院重点项目,编号CBY13-A-ZD05。
