摘要
目的探讨ABCB1基因(多药耐药基因)C3435T多态性与儿童急性淋巴细胞白血病发病情况的相关性。方法选取60例急性淋巴细胞白血病患儿作为研究对象,依照中国抗癌协会小儿专业委员会急性淋巴细胞白血病2015方案(CCCG.ALL.2015)的危险度分级标准,将患儿分为低危组(n=25)和中高危组(n=35)。采集患儿的骨髓液1.5 ml提取DNA,应用DNA测序技术检测ATP结合盒式蛋白B1基因3435位点多态性,并分析ABCB1基因C3435T多态性与儿童急性淋巴细胞白血病发病情况的相关性。结果2组患儿ABCB1基因C3435T位点中CT、CC点位对比无明显差异(P>0.05),TT点位对比差异显著(P<0.05)。不同点位ABCB1基因C3435T的急性淋巴细胞白血病患儿生存情况对比无明显差异(P>0.05)。60例急性淋巴细胞白血病患儿ABCB1基因多态性不同点位患儿的性别、年龄、血小板减少患儿、中性粒细胞减少患儿对比均无明显差异(P>0.05),ABCB1基因多态性不同点患儿的血红蛋白减少情况对比差异显著(P>0.05)。儿童急性淋巴细胞白血病发病情况与血小板、血红蛋白、中性粒细胞、C3435T等位基因、危险度均有明显关系(P<0.05)。结论ABCBl基因C3435T多态性与儿童ALL可能存在着一定关系,具有一定的参考价值。
Objective To investigate the correlation between C3435 T polymorphism of ABCB1 gene(multidrug resistance gene)and childhood acute lymphoblastic leukemia.Methods 60 children with acute lymphoblastic leukemia were selected as research subjects.According to the risk classification standard of cccg.All.2015,the children were divided into low-risk group(n=25)and medium high-risk group(n=35),The association of ab535 gene polymorphism with the incidence of acute leukemia was analyzed by cbt-435 DNA sequencing.Results There was no significant difference in CT and CC in C3435 T locus of ABCB1 gene between the 2 groups(P>0.05),but there was significant difference in TT between the 2 groups(P<0.05);there was no significant difference in the survival of children with acute lymphoblastic leukemia at different sites of ABCB1 gene C3435 T(P>0.05);the gender and year of ABCB1 gene polymorphism in 60 children with acute lymphoblastic leukemia were different,There was no significant difference in age,thrombocytopenia and neutropenia(P>0.05),but there was significant difference in hemoglobin reduction in children with different abcbl gene polymorphisms(P<0.05);the incidence of childhood acute lymphoblastic leukemia was significantly related to platelet,hemoglobin,neutrophil,C3435 T allele and risk(P<0.05).Conclusion The C3435 T polymorphism of ABCB1 gene may have a certain relationship with childhood ALL,which has certain reference value.
作者
孙元杰
牛银萍
关红梅
SUN Yuanjie;NIU Yinping;GUAN Hongmei(Nanyang Second People's Hospital,Nanyang,473000)
出处
《实用癌症杂志》
2021年第8期1242-1244,1248,共4页
The Practical Journal of Cancer
基金
2019年度南阳市医学科技攻关项目(编号:KJGG131)。
