摘要
目的:观察梓醇对甲状腺功能亢进小鼠模型肝功能及氧化应激相关指标的影响,探讨梓醇防治甲状腺功能亢进肝损伤的作用机制。方法:将40只健康BALB/c雌性小鼠分为空白组、模型组、甲巯咪唑组(西药组)、梓醇组,每组10只。除空白组外,其余各组制备甲状腺功能亢进模型,模型制备成功后,模型组及空白组给予蒸馏水灌胃,西药组及梓醇组给予相应的药物灌胃。给药结束后摘眼球取血,采用酶联免疫吸附法检测血清中促甲状腺激素(TSH)、三碘甲状腺原氨酸(T3)、四碘甲状腺氨酸(T4)、谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(ALP)的含量;采用比色法检测肝脏组织中丙二醛(MDA)、超氧化物歧化酶(SOD)的活力单位;生化法得出过氧化氢酶(CAT)含量。结果:与空白组比较,模型组小鼠血清TSH含量降低(P<0.05),血清T3、T4、AST、ALT、ALP含量及肝脏组织MDA、SOD、CAT含量升高(P<0.05);与模型组比较,梓醇组及西药组小鼠血清TSH含量升高(P<0.05),血清T3、T4、AST、ALT、ALP含量及肝脏组织MDA、SOD、CAT含量降低(P<0.05);与西药组比较,梓醇组小鼠血清TSH含量较高(P<0.05),血清T3、T4、AST、ALT、ALP含量及肝脏组织MDA、SOD、CAT含量较低(P<0.05)。结论:梓醇治疗甲状腺功能亢进的效果优于西药,更能降低肝脏氧化应激损伤。
Objective:To observe the effect of catalpol on the liver function and the indexes related to oxidative stress in mice models with hyperthyroidism,and to discuss the mechanism of catalpol on prevention and treatment of hyperthyroidism complicated with the liver injury. Methods:A total of 40 healthy BALB/c female mice were divided into the blank group,the model group,the methimazole group(the western medicine group),and the catalpol group,10 mice in each group. Except for the blank group,the models with hyperthyroidism in the other groups were prepared. After successful establishment,the model group and the blank group were given gastric perfusion with distilled water. The western medicine group and the catalpol group were given gastric perfusion with corresponding medicine. After medication, eyeballs were extracted and blood was drawn. Enzyme-linked immunosorbent assay was applied to detect the contents of thyroid stimulating hormone(TSH),triiodothyronine(T3),Tetraiodothyronine(T4),aspartate transaminase(AST),alanine transaminase(ALT),and alkaline phosphatase(ALP) in serum. Colorimetry was used to detect activity units of malondialdehyde(MDA) and superoxide dismutase(SOD). Biochemistry was used to detect the content of catalase(CAT). Results:Compared with those in the blank group,the content of TSH in serum in the model group was decreased(P<0.05);contents of T3,T4,AST,ALT,and ALP in serum as well as contents of MDA,SOD,and CAT in the liver tissue were increased(P<0.05). Compared with those in the model group,contents of TSH in serum in the catalpol group and the western medicine group were increased(P<0.05);contents of T3,T4,AST,ALT,and ALP in serum as well as contents of MDA,SOD,and CAT in the liver tissue were decreased(P<0.05). Compared with those in the western medicine group,content of TSH in serum in the catalpol group was increased(P<0.05);contents of T3,T4,AST,ALT,and ALP in serum as well as contents of MDA,SOD,and CAT in the liver tissue were decreased(P<0.05). Conclusion: Catalpol has a better effect than western medicine in the treatment of hyperthyroidism,which can further relieve oxidative stress injury in the liver.
作者
杨鹿鸣
董士中
马艳庆
YANG Luming;DONG Shizhong;MA Yanqing
出处
《新中医》
CAS
2021年第17期12-16,共5页
New Chinese Medicine
基金
河南省2015年科技发展计划项目(152102310358)。
关键词
甲状腺功能亢进
梓醇
肝功能
氧化应激
动物实验
小鼠
Hyperthyroidism
Catalpol
Liver function
Oxidative stress
Animal experiment
Mice
