摘要
通过Illumina Hiseq4000高通量测序平台,采用PE150测序策略研究了玉蜀黍平脐蠕孢在链霉菌发酵液胁迫下(S组)的转录组变化,共得到128613786个clean reads,筛选出4559个差异基因.与无发酵液的对照组相比,S组中有2283个基因表达上调,2276个基因表达下调;从中挑选出表达量差异最大的22个基因作为内生链霉菌调控拮抗的拟关键基因,这些基因多涉及氨基酸合成和代谢以及信号转导途径,说明链霉菌发酵液对玉蜀黍平脐蠕孢的氨基酸生物合成和MAPK信号通路相关基因的影响显著;采用实时荧光定量PCR验证其中5个基因的表达量,其趋势与转录组分析结果基本一致.
Transcriptome changes of Bipolaris maydis under the stress of Streptomyces sp.fermentation broth were evaluated using PE150 sequencing strategy under Illumina Hiseq4000 high-throughput sequencing platform.A total of 128613786 clean reads were obtained,and 4559 differential genes were screened.Compared with the CK group,2283 genes were up-regulated and 2276 genes were down-regulated.Then 22 genes,witnessing the most changes were designated as the putative key genes in playing antagonist roles against B.maydis.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that most of the genes were involved in amino acid synthesis and metabolism,and signal transduction pathways,suggesting that the fermentation broth of Streptomyces sp.had significant effects on the genes related to amino acid biosynthesis and MAPK signaling pathway in B.maydis.Meanwhile real-time fluorescent quantitative PCR was conducted to verify the expressions of the 5 genes,and their expression trends were consistent with the results of transcriptome analysis.
作者
黄在兴
宋昭昭
梅兰
李曼
刘朋虎
苏德伟
林占熺
鲁国东
HUANG Zaixing;SONG Zhaozhao;MEI Lan;LI Man;LIU Penghu;SU Dewei;LIN Zhanxi;LU Guodong(National Engineering Research Center of JUNCAO Technology,Fujian Agriculture and Forestry University;College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2021年第5期686-693,共8页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
中央引导地方科技发展专项(2018L3003)。
关键词
生物防治
RNA-SEQ
高通量测序
差异基因
biological control
RNA-sequencing
high-throughput sequencing
differential gene
