摘要
研究瓦山锥(Castanopsis ceratacantha)叶子中的化学成分及其酪氨酸酶抑制活性。综合采用Sephadex LH-20、Toyopearl HW-40F、Diaion HP20SS等多种柱色谱和高效液相色谱等分离手段对瓦山锥叶子80%乙醇提取物进行化学成分的分离纯化,通过波谱数据分析并结合文献对照鉴定化合物结构,并对瓦山锥叶子中的特征化化合物进行酪氨酸酶抑制活性筛选。在前期研究中,本课题组已从瓦山锥叶子80%乙醇提取物分离得到11个化合物,在此研究基础上继续从瓦山锥叶子80%乙醇提取物中分离得到17个化合物,分别鉴定为原儿茶酸(1)、6-O-没食子酰基熊果苷(2)、3-甲氧基-4-羟基苯酚1-O-β-D-(6′-O-没食子酰基)吡喃葡萄糖苷(3)、瓦隆酸双内酯(4)、1,2,3,4,6-五-O-没食子酰基-β-D-吡喃葡萄糖(5)、长梗马兜铃素(6)、木麻黄鞣亭(7)、praecoxin A(8)、栗木素(9)、栎木素(10)、chinquapinic acid(11)、栎木鞣花素(12)、栗木鞣花素(13)、castacrenin D(14)、山奈酚(15)、异槲皮素(16)、aviculin(17)。所有化合物皆为首次从瓦山锥植物中分离得到,实验结果显示化合物14具有较强的酪氨酸酶抑制活性,其他化合物也具有较好的抑制活性。
Study the chemical constituents and their tyrosinase inhibitory activity in the leaves of Castanopsis ceratacantha.The compounds were isolated and purified by Sephadex LH-20,Toyopearl HW-40F,Diaion HP20SS and other column chromatography and HPLC.The structures of compounds were elucidated by spectroscopic data and comparison with literatures.The characteristic compounds in the leaves of C.ceratacantha were screened for tyrosinase inhibitory activity.In the previous research,our group had isolated 11 compounds from the 80%ethanol extract of C.ceratacantha.On the basis of this research,17 compounds were obtained and their structures were identified as protocatechuic acid(1),6-O-galloylarbutin(2),3-methoxy-4-hydroxyphenol 1-O-β-D-(6′-O-galloyl)glucopyranoside(3),valoneic acid dilactone(4),1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose(5),pedunculagin(6),casuarictin(7),praecoxin A(8),castalin(9),vescalin(10),chinquapinic acid(11),vescalagin(12),castalagin(13),castacrenin D(14),kaempferol(15),isoquercitrin(16),aviculin(17).All the compounds were isolated from this plant for the first time.Experimental results show that compound 14 has strong tyrosinase inhibitory activity,and other compounds also have better inhibitory activity.
作者
王亚凤
李思雯
何瑞杰
阳丙媛
黄永林
WANG Ya-feng;LI Si-wen;HE Rui-jie;YANG Bing-yuan;HUANG Yong-lin(Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization,Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006,China;Guilin Medical University,Guilin 541004,China)
出处
《天然产物研究与开发》
CAS
CSCD
2021年第9期1499-1505,共7页
Natural Product Research and Development
基金
广西自然科学基金(2018GXNSFAA281078)
广西创新驱动发展专项(桂科AA18118015)
中央引导地方科技发展专项(桂科20111010)
广东省重点领域研发计划(2020B1111110003)。
关键词
瓦山锥
化学成分
单宁
结构鉴定
酪氨酸酶抑制剂
Castanopsis ceratacantha
chemical constituents
tannins
structural identification
tyrosinase inhibitor
