摘要
为探究烟草花粉不同发育时期内含物和花粉活力的变化规律,本研究以烟草品种‘K326’新鲜花药为材料,检测花粉不同发育时期可溶性糖、可溶性淀粉、可溶性蛋白质等内含物以及鲜干重、ATP酶活性和花粉活力变化。结果表明:烟草品种‘K326’花粉的干物质积累主要在时期5及其之前完成,时期5以后主要进行花粉内部的物质转化过程,且在此过程中花药会部分脱水,使得花粉耐干燥,提高对外部环境的抗性;利用离体萌发法检测花粉活力,发现花冠变白膨大,顶端显红色时花粉开始有活力,并在花药刚刚开裂即将散粉时达到最大;花粉内含物变化比较复杂,可溶性糖和可溶性淀粉含量呈现“N”型增长变化,总氨基酸含量呈现“S”型增长变化,可溶性蛋白质含量先下降后上升,脯氨酸含量先上升后下降,ATP酶活性总体处于较高水平,花粉成熟时显著上升。由此可见,可溶性糖、可溶性淀粉、可溶性蛋白质、总氨基酸含量和ATP酶活性对花粉活力有正向作用。
In order to investigate the changes in the inclusions and pollen vitality of tobacco pollen at different developmental stages,'K326'fresh anther was used as the material to determine the soluble sugar,soluble starch,soluble protein,fresh and dry weight,ATPase activity and pollen vitality of pollen at different developmental stages.The results showed that the dry matter accumulation of tobacco pollen was mainly completed in Stage 5 and before,and the material conversion process inside the pollen was mainly carried out after Stage 5,and during this process,the anthers would be partially dehydrated,improving the pollen's resistance to drying and adapt to the external environment.The pollen vitality in fresh anther was detected by vitro-germination method,and it was found that the pollen vitality appeared when the corolla became white and swelled,and the tip appeared red;And it would reached the maximum when the anthers were just cracked and the powder was loose.The content of soluble sugar and soluble starch showed an"N"growth change,and the total amino acid content showed an"S"type growth change.The soluble protein content decreased first and then increased,and the proline content increased first and then decreased.ATPase activity is generally at a relatively high level,and it increases significantly when pollen matures.It can be seen that soluble sugar,soluble starch,soluble protein,total amino acid content and ATPase activity have positive effects on pollen vitality.
作者
许杰
潘磊
陈云松
邓盛斌
肖江海
马文广
Xu Jie;Pan Lei;Chen Yunsong;Deng Shengbin;Xiao Jianghai;Ma Wenguang(Yuxi Zhongyan Tobacco Seed Company,Yuxi,653100)
出处
《分子植物育种》
CAS
北大核心
2021年第17期5861-5868,共8页
Molecular Plant Breeding
基金
云南省烟草公司重点科技项目(2018530000241034)资助。
关键词
烟草花粉
花粉活力
内含物
Tobacco pollen
Pollen vitality
Inclusions
