摘要
目的观察夹脊穴电针对实验性脊髓损伤(SCI)后中枢性疼痛模型大鼠自发痛行为学和痛超敏现象的影响。方法将30只SPF级雄性SD大鼠(56日龄,体重230~240 g)采用随机数字表法分为空白对照组、SCI组和夹脊穴组,每组10只。其中SCI组和夹脊穴组采用Allen’s打击法建立脊髓L1节段实验性SCI后中枢性疼痛模型,空白对照组不予手术造模。夹脊穴组取T8~T12夹脊穴直刺后接电30 min,1次/d,共14 d,空白对照组和SCI组仅固定(30 min)。治疗期间观察大鼠自发痛行为学;于治疗14 d后采用单极经皮层电刺激记录脊髓运动诱发电位(scMEP);采用刺激记录L1段体感诱发电位(SEP);von Frey hair法测定机械痛阈;热板法测定热痛敏;以Basso Beattie Bresnahan(BBB)评分和斜板实验评价治疗效果。结果夹脊穴组自发嘶叫、搔抓、舔咬、自噬发生次数低于SCI组,差异有统计学意义(P<0.05)。与SCI组比较,夹脊穴组scMEP早成份N1和N2波峰值升高、SEP和P波、N波潜伏期均缩短,夹脊穴组损伤平面下端躯干对非伤害性机械性轻压和轻触及引起50%前爪抬足的疼痛反应阈值和热痛阈提高,热缩足潜伏期延长,痛超敏发生率降低,BBB评分提高,斜板倾斜度增大,差异有统计学意义(P<0.05)。结论夹脊穴电针可提高痛阈、疏通“督脉损伤”之经络进而改善自发痛和痛超敏现象,这有利于促进神经传导及运动功能恢复。
Objective To observe the effects of jiájǐpoint electroacupuncture on spontaneous pain behavior and hyperalgesia in central pain model rats after experimental spinal cord injury(SCI).Methods Thirty SPF male SD rats(56 days of age,weight 230-240 g)were divided into blank control group,SCI group and jiájǐpoint group by random number table method,with ten rats in each group.In the SCI group and the jiájǐpoint group,Allen’s percussion method was used to establish the experimental SCI central pain model at the L1 segment of the spinal cord,and the blank control group was not operated to create the model.The jiájǐpoint group was treated with direct acupuncture at T8-T12 jiájǐpoint for 30 min,once a day,for 14 days,while the blank control group and the SCI group were only fixed(30 min).The spontaneous pain behavior of rats was observed during treatment.After 14 days of treatment,Spinal cord motor evoked potential(scMEP)was recorded by unipolar transcortical electrical stimulation.Somatosensory evoked potential(SEP)in L1 was recorded by stimulation.Mechanical pain threshold was determined by von Frey hair method.The hot plate method was used to determine the hyperalgesia.Basso Beattie Bresnahan(BBB)score and oblique plate test were used to evaluate the treatment effect.Results The incidence of spontaneous screaming,scratching,licking,biting and autophagy in the jiájǐpoint group was lower than that in the SCI group,and the difference was statistically significant(P<0.05).Compared with the SCI group,N1 and N2 peaks of early components of scMEP in the jiájǐpoint group were increased,and the latency of SEP,P and N waves were shortened,the pain response threshold of the lower trunk of the injured plane to non-invasive mechanical light pressure and light touch and 50%forepaw lifting in the jiájǐpoint group were increased,the Paw withdrawal latency of the jiájǐpoint group was increased,the paw withdrawal latencies were prolonged,the incidence of hyperalgesia was decreased,the BBB score was increased,and the inclination of inclined plate was increased,with statistical significance(P<0.05).Conclusion Electroacupuncture at jiájǐpoint can improve the pain threshold,dredge the meridians of“governor channel injury”and improve the spontaneous pain and hyperalgesia,which is conducive to the recovery of nerve conduction and motor function.
作者
左薇
穆敬平
许明军
ZUO Wei;MU Jingping;XU Mingjun(Department of Acupuncture Treatment,Taihe Hospital,Affiliated Hospital of Hubei University of Medicine,Hubei Province,Shiyan442000,China;Sleep Psychosomatic Medicine Center,Taihe Hospital,Affiliated Hospital of Hubei University of Medicine,Hubei Province,Shiyan442000,China)
出处
《中国医药导报》
CAS
2021年第27期17-20,共4页
China Medical Herald
基金
湖北省卫健委面上项目(WJ2017F072)。
关键词
夹脊穴
电针
实验性脊髓损伤
中枢性疼痛
自发痛行为学
痛超敏现象
督脉损伤
Electroacupuncture
jiájǐpoint
Experimental spinal cord injury
Central pain
Spontaneous pain behavior
Hyperalgesia
Governor vessel injury
