摘要
目的探究结直肠癌组织及血清中微小RNA-107(miRNA-107)相对表达水平与临床病理特征及预后的关系。方法选取2017年11月至2019年4月期间于该院收治的60例患者作为试验组,根据疾病进展分为早期癌症组及进展期癌组,每组30例。并选取同期体检健康的20例就诊者为对照组,20例结直肠息肉患者为息肉组。比较各组受试者miRNA-107相对表达水平的差异及与临床特征的相互关系,绘制患者无进展生存曲线,评估miRNA-107相对表达水平与结直肠癌患者的预后相关性。结果息肉组、早期癌症组和进展期癌组血清miRNA-107水平明显高于对照组(P<0.05),早期癌症组和进展期癌组血清miRNA-107水平明显高于息肉组(P<0.05),进展期癌组血清miRNA-107水平明显高于早期癌症组(P<0.05)。进展期癌组肿瘤组织miRNA-107水平明显高于早期癌症组(P<0.05)。Spearman相关分析表明,试验组血清、肿瘤组织miRNA-107相对表达水平呈正相关(r=0.446,P<0.05)。血清、肿瘤组织miRNA-107相对表达水平与患者的无进展生存期(PFS)相关,其相对表达水平越高,预后越差。血清、肿瘤组织miRNA-107相对表达水平在癌胚抗原(CEA)≤3.52 ng/L和CEA>3.52 ng/L患者之间差异有统计学意义(P<0.05)。血清、肿瘤组织中miRNA-107阳性及CEA>3.52 ng/L可作为影响结直肠癌患者PFS的独立预后危险因素(P<0.05)。结论miRNA-107在结直肠癌患者的血清、肿瘤组织中均高表达,其相对表达水平无明显差异,且相对表达水平越高则预后越差,可作为结直肠癌诊断、预后的参考指标。
Objective To investigate the relationship between the expression of microRNA-107(miRNA-107)in colorectal cancer tissues and serum and its clinicopathological characteristics and prognosis.Methods A total of 60 patients admitted to the hospital from November 2017 to April 2019 were taken as the experimental group.They were divided into early cancer group and middle and advanced cancer group according to disease progression,with 30 cases in each group.20 healthy patients were selected as the control group and 20 patients with colorectal polyps was taken as the polyp group.The levels of miRNA-107 in each group were compared and their correlation with clinical features were compared.No progress survival curve was drawn and the correlation between the level of miRNA-107 and the prognosis of colorectal cancer patients was evaluated.Results The level of serum miRNA-107 in polyp group,early cancer group and advanced cancer group was significantly higher than that in control group(P<0.05),the level of serum miRNA-107 in early cancer group and advanced cancer group was significantly higher than that in polyp group(P<0.05),and the level of serum miRNA-107 in advanced cancer group was significantly higher than that in early cancer group(P<0.05).The level of miRNA-107 in tumor tissues in advanced cancer group was significantly higher than that in early cancer group(P<0.05).Spearman analysis showed that there was a positive correlation between RNA-107 levels in serum and tumor tissue(r=0.446,P<0.05).The expression level of miRNA-107 in serum and tumor tissue was correlated with the progression-free survival(PFS)of patients.The higher the level,the worse the prognosis.The relative expression levels of miRNA-107 in serum and tumor tissues were significantly different between patients with Carcinoembryonic antigen(CEA)≤3.52 ng/L and CEA>3.52 ng/L(P<0.05).MiRNA-107 positive in serum and tumor tissues and CEA>3.52 ng/L could be used as independent prognostic risk factors for PFS in patients with colorectal cancer(P<0.05).Conclusion miRNA-107 is highly expressed in the serum and tumor tissues of patients with colorectal cancer without significant difference,and when the miRNA-107 level is higher,the prognosis is worse,which can be used as a reference index for the diagnosis and prognosis of colorectal cancer.
作者
张肖丽
张月晓
李炳庆
李萍
陈健
ZHANG XiaoLi;ZHANG YueXiao;LI Bingqing;LI Ping;CHEN Jian(Department of Gastroenterology,Affiliated Hospital of Chengde Medical College,Chengde,Hebei 067000,China)
出处
《国际检验医学杂志》
CAS
2021年第19期2379-2383,共5页
International Journal of Laboratory Medicine
基金
承德市科技支撑计划项目(201701A037)。
