摘要
目的分析经肾动脉移植骨髓间充质干细胞(BMSC)对慢性肾病(CKD)大鼠肾脏炎症的影响。方法取37只雄性Sprague-Dawley(SD)大鼠,其中2只4周龄用作体外分离BMSC,8只为正常对照组(N组),27只制作CKD模型。建模中3只死亡,24只建模成功后,随机分为CKD组(n=8)、经肾动脉移植BMSC组(A组,n=8)和经尾静脉移植BMSC组(V组,n=8)。BMSC移植后7、14 d,检测各组大鼠肾功能指标,制作肾脏病理切片,苏木精-伊红(HE)染色观察肾脏病理形态学变化,免疫组化染色分析肾组织炎性细胞表达。结果CKD组、A组、V组与N组相比,BMSC移植后血肌酐、血尿素氮和24 h尿蛋白均显著升高(P<0.01)。移植后7、14 d,V组、A组血肌酐均较CKD组降低(P<0.01),A组在7 d时下降更为显著(P<0.01);移植后7 d,V组、A组血尿素氮较CKD组均显著下降(P<0.01),A组变化更为显著(P<0.01),移植后14 d,V组、A组较CKD组稍降低,A组变化更为显著(P<0.01);移植后7、14 d,V组、A组24 h尿蛋白较CKD组均显著降低(P<0.01),A组下降更为显著(P<0.01)。HE染色病理结果显示A组、V组炎性浸润均较CKD组减轻,A组仅有少量肾间质炎性细胞浸润,萎缩肾小球数目减少,肾小管扩张情况有不同程度改善。免疫组化结果显示CKD组大鼠CD3、CD20呈强阳性或阳性表达,A组、V组大鼠肾间质CD3、CD20表达较CKD组减轻,但A组较V组减轻更为明显。结论BMSC通过抑制免疫介导性炎症修复受损肾脏组织。经肾动脉移植效果优于经尾静脉移植。
Objective To analyze the effect of bone marrow mesenchymal stem cells(BMSC) transplantation via renal artery on the renal inflammation in experimental rats with adriamycin-induced chronic kidney disease(CKD).Methods A total of 37 male Sprague-Dawley(SD)rats were used in this study.Of the 37 rats,2 were 4 weeks old and were used for isolating and culturing BMSC in vitro,8 were used as the normal control group(N group),and 27 were used to establish CKD model.Three rats died during the course of modelling,successful modeling was achieved in the remaining 24 rats,which were randomly divided into CKD group(n=8),BMSC transplantation via renal artery group(group A,n=8)and BMSC transplantation via caudal vein group(group V,n=8).In each group,the renal functions were tested at 7 and 14 days after BMSC transplantation,the renal pathological sections together with hematoxylin-eosin(HE)staining were made to microscopically observe the pathomorphological changes of kidney,and the immunohistochemical staining method was adopted to analyze the expression of inflammatory cells in renal tissues.Results Compared with group N,in CKD group,group A and group V the levels of serum creatinine,blood urea nitrogen and 24-h urinary protein were significantly elevated after BMSC transplantation(P<0.01).At 7 and 14 days after BMSC transplantation,the level of serum creatinine in group V and group A was lower than that in CKD group(P<0.01),and it was remarkably lower in group A at 7 days after BMSC transplantation(P<0.01).Seven days after BMSC transplantation,a striking reduction in the level of blood urea nitrogen was observed in group V and group A(P<0.01),which was more prominent in group A(P<0.01).Fourteen days after BMSC transplantation,the level of blood urea nitrogen in group V and group A was slightly lower than that in CKD group,which was more prominent in group A(P<0.01).Pathological examination showed that inflammatory infiltration in group A and group V was more obviously alleviated than that in CKD group,as microscopical examination showed that in group A only small amount of renal interstitial inflammatory cell infiltration was observed,the number of atrophic glomeruli was obviously reduced and the dilatation of renal tubules was much improved.Immunohistochemical staining examination revealed that in CKD group the expressions of CD3 and CD20 in renal interstitial tissues were strong positive or positive,while in group A and group V the expressions of CD3 and CD20 in renal interstitial tissues were slight,which in group A was much slighter than in group V.Conclusion BMSC can repair damaged kidney tissue through suppressing immune-mediated inflammation.In performing BMSC transplantation therapy,via renal artery path is superior to via caudal vein path in achieving better clinical effect.
作者
李天祎
杨扬
万珊杉
杨素萍
李嘉琦
王家平
LI Tianyi;YANG Yang;WAN Shanshan;YANG Suping;LI Jiaqi;WANG Jiaping(Department of Radiology,Second Affiliated Hospital of Kunming Medical University,Kunming,Yunnan Province 650101,China)
出处
《介入放射学杂志》
CSCD
北大核心
2021年第9期915-919,共5页
Journal of Interventional Radiology
基金
国家自然科学基金(81860144)
昆明医科大学研究生创新基金(2020S051)。
关键词
骨髓间充质干细胞
慢性肾病
肾脏炎症
肾动脉
bone marrow mesenchymal stem cell
chronic kidney disease
renal inflammation
renal artery
