摘要
目的观察左氧氟沙星和莫西沙星治疗豚鼠实验感染鼠疫的疗效。方法参照《中华人民共和国国家药典》成人剂量,用施新猷的动物折算系数法换算成豚鼠每日每千克体重用药剂量[左氧氟沙星40 mg/(kg·d),莫西沙星32 mg/(kg·d)],用灭菌生理盐水稀释成水剂后,以皮下注射法治疗鼠疫菌141株感染24 h后的实验动物。连续用药9 d后停药,经医学观察9 d后处死,自然死亡及处死动物解剖取脏器进行鼠疫细菌学检验。结果左氧氟沙星和莫西沙星治疗组实验动物全部存活,存活率均为100%;存活动物处死后,解剖取脏器进行细菌学检验,结果均为阴性。对照组动物于感染后3~5 d全部死亡,并从各脏器中分离到鼠疫菌,均为特异性死亡。结论左氧氟沙星和莫西沙星对豚鼠实验感染鼠疫的治疗效果均有效,特殊情况下可替代链霉素用于鼠疫的预防和治疗。
To observe the efficacy of levofloxacin and moxifloxacin in the treatment of experimental plague in guinea pigs,according to the National Pharmacopoeia of the People’s Republic of China,we converted the daily doses of levofloxacin and moxifloxacin into per kg body weight for guinea pigs according to the animal conversion coefficient[levofloxacin 40 mg/(kg·d)and moxifloxacin 32 mg/(kg·d)].The two drugs were diluted into an aqueous solution in sterilized saline and subcutaneously injected into the experimental animals infected with 141 strains of Yersinia pestis after 24 hours.All experimental animals were given continuous treatment for 9 days,and then the drug administration was stopped.The animals were then sacrificed after 9 days of observation.The animals that died of infection or were sacrificed were dissected,and their organs were subjected to plague bacteriological examination.All experimental animals in the levofloxacin and moxifloxacin treatment groups survived(survival rates of 100%).All surviving animals were sacrificed and dissected,and organs were collected for plague bacteriological examination and culturing.The results were all negative;the experimental animals in the control groups died 3~5 days after infection,and Yersinia pestis was isolated from each animal.Thus,these deaths were considered to be due to Yersinia pestis infection.Both levofloxacin and moxifloxacin were effective in the treatment of guinea pigs infected with Yersinia pestis and thus can substitute for streptomycin in plague prevention and treatment.
作者
李胜
何建
辛有全
杨晓艳
靳娟
张琪
杨建国
熊浩明
柏吉祥
代瑞霞
LI Sheng;HE Jian;XIN You-quan;YANG Xiao-yan;JIN Juan;ZHANG Qi;YANG Jian-guo;XIONG Hao-ming;BAI Ji-xiang;DAI Rui-xia(Qinghai Institute for Endemic Disease Prevention and Control,Xining 811602,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2021年第9期858-860,共3页
Chinese Journal of Zoonoses
基金
青海省科技厅基础研究计划项目(No.2019-ZJ-7074)
国家卫生健康委鼠疫防治研究重点实验室(共建)(No.2019PT310004)
青海省鼠疫防控及研究重点实验室(No.2021-ZJ-Y15)联合资助。
关键词
抗菌药物
治疗
动物实验
鼠疫
疗效
antimicrobial agents
treatment
animal experiments
the plague
the curative effect
