摘要
NPF基因家族是植物体内普遍存在的硝酸盐转运蛋白,同时负责硫苷、多肽、钾盐、植物激素等物质的运输。为了明确白菜NPF基因家族的功能和进化关系,利用生物信息学方法鉴定了白菜的NPF基因家族成员,并对NPF基因家族蛋白进行了理化性质、二级结构、亚细胞定位、保守基序分布以及与拟南芥的进化关系分析,对白菜NPF基因家族成员进行了基因结构以及染色体定位分析。结果表明,白菜NPF基因家族共有72个成员,编码蛋白质的氨基酸数量在497~1 117个,分子质量在54.49~123.21 ku,等电点在5.72~9.47,大部分为稳定的碱性蛋白。二级结构以α-螺旋为主,其次是无规则卷曲,亚细胞定位预测均在质膜上。系统进化树将白菜NPF基因家族蛋白分为8个亚族,其保守性较高。白菜NPF基因家族成员大部分含有4个CDS序列。72个NPF基因家族成员不均匀地分布在白菜10条染色体上。
The NPF gene family is a nitrate transporter ubiquitous in plants,and is also responsible for transporting glucosinolates,peptides,potassium salts,and plant hormones.In order to clarify the function and evolutionary relationship of the NPF gene family of Chinese cabbage,bioinformatics methods were used to identify the members of the NPF gene family of Chinese cabbage,and the physical and chemical properties,secondary structure,subcellular location,conserved motif distribution and evolutionary relationship with Arabidopsis were analyzed for the NPF gene family proteins.The gene structure and chromosome location of the NPF gene family members of Chinese cabbage were also analyzed.The results showed that there were 72 members in the NPF gene family of Chinese cabbage,the number of encoded amino acids ranged from 497 to 1117,the molecular weight ranged from 54.49 to 123.21 ku,the isoelectric point ranged from 5.72 to 9.47,and most of them were stable basic proteins.The secondary structure was dominated by alpha helix,followed by random coils.Subcellular localization prediction showed that they were all on the plasma membrane.The phylogenetic tree divided Chinese cabbage NPF gene family proteins into 8 sub-families,which were highly conserved.Most of the NPF gene family members in Chinese cabbage contained 4 CDS sequences.The 72 NPF gene family members were unevenly distributed on 10 chromosomes of Chinese cabbage.
作者
马嘉俊
吴英华
李璿
李梅兰
侯雷平
MA Jiajun;WU Yinghua;LI Xuan;LI Meilan;HOU Leiping(College of Horticulture,Shanxi Agricultural University/Collaborative Innovation Center for Improving Quality and Increasing Profits of Protected Vegetables in Shanxi,Taigu 030800,China)
出处
《河南农业科学》
北大核心
2021年第9期117-127,共11页
Journal of Henan Agricultural Sciences
基金
山西省重点研发计划项目(201703D211001-04-01,201803D221005-8)。
关键词
白菜
NPF基因家族
生物信息学
理化性质
保守基序
Chinese cabbage
NPF gene family
Bioinformatics
Physical and chemical properties
Conservative motif
