摘要
目的:探讨Egr-1在单眼形觉剥夺性弱视幼猫视皮质中的表达及其意义。方法:将30只3周龄健康幼猫随机分成形觉剥夺组与对照组,每组15只。饲养于自然光照下,将剥夺组幼猫通过黑色不透明遮盖布遮盖右眼。于遮盖前和遮盖后的第1、3、5周对所有幼猫行图形视觉诱发电位(PVEP)检查;遮盖后第5周PVEP检测完成后,处死幼猫,根据《猫解剖彩色图谱》,取视皮质,行HE染色和免疫组织化学检测,利用Image-Pro Plus统计并分析其阳性细胞数与平均光密度值。结果:PVEP检测显示,遮盖后第3周和第5周,剥夺组右眼P100波潜伏期高于剥夺组左眼(P<0.05)和对照组右眼(P<0.05),振幅下降(P<0.05)。免疫组化显示,Egr-1在两组幼猫视皮质中均有阳性表达;遮盖5周后剥夺组视皮质中Egr-1阳性细胞数与平均光密度值均低于对照组(P<0.05)。结论:单眼形觉剥夺性弱视会导致视皮质内的Egr-1蛋白表达量下降,促进弱视的发生发展。
Objective:To explore the expression and significance of Egr-1 in the visual cortex of kitten with monocular deprivation amblyopia.Methods:A total of 30 healthy kittens aged 3 weeks were selected,and they were randomly divided into deprivation group and control group,with 15 kittens in each group.Raised in natural light,and cover the right eye of the deprived kittens with a black opaque covering cloth.Pattern visual evoked potentials(PVEP)were measured before and at the 1,3 and 5 w after covering in all kittens.PVEP test was completed at 5 w after covering,and kittens were killed.According to Atlas of Feline Anatomy For Veterinarians,the visual cortex was taken and detected by HE staining and immunohistochemistry.The number of positive cells and the average optical density were analyzed by Image-Pro Plus.Results:PVEP detection showed that at the 3 and 5 w after covering,the P100 wave latency in the right eye of deprivation group was higher than that in the left eye of deprivation group(P<0.05)and the right eye of control group(P<0.05),while the amplitude decreased(P<0.05).Immunohistochemistry showed that Egr-1 was positive in the visual cortex of both groups.And the number of Egr-1 positive cells and average optical density in visual cortex of deprivation group were lower than those of normal group after 5 w of covering(P<0.05).Conclusion:Monocular form deprivation amblyopia can lead to the decrease of Egr-1 protein expression in visual cortex,and promote the occurrence and development of amblyopia.
作者
范浩博
唐秀平
杨丽源
宋唯琦
王英
陈思宇
邹云春
FAN Hao-bo;TANG Xiu-ping;YANG Li-yuan;SONG Wei-qi;WANG Ying;CHEN Si-yu;ZOU Yun-chun(Department of Optometry,North Sichuan Medical College,Nanchong 637000,Sichuan,China)
出处
《川北医学院学报》
CAS
2021年第9期1232-1236,共5页
Journal of North Sichuan Medical College
基金
四川省科技厅计划项目(2015JY0263)
四川省医学科研课题(S18034)
南充市科技项目市校合作战略合作专项(18SH20386)。
关键词
早期生长反应因子-1
弱视
形觉剥夺
视皮质
幼猫
Early growth responsive gene-1(Egr-1)
Amblyopia
Form deprivation
Visual cortex
Kitten
