摘要
组织培养是竹类植物快速繁育的重要手段之一。基于CNKI和WOS数据库,利用文献计量分析及统计学手段对1960-2020年间竹组织培养研究相关论文的发表量、时间、地区、属种、组培类型以及外植体选择等方面进行了深入研究。结果表明:(1)发展中国家是竹组织培养研究的主要国家,与竹种分布、人口结构和经济技术发展水平相关,研究作者群体和机构呈现“小聚集、大分散”特征;(2)研究竹种主要集中在牡竹属、簕竹属和刚竹属,与竹种特性、用途和组培技术有关;(3)竹组织培养类型主要为以芽繁芽、胚培养及愈伤组织再生植株3种;(4)消毒剂氯化汞、乙醇和次氯酸钠溶液,培养基MS、3/4MS和1/2MS,生长调节剂BA、IBA和NAA等对竹组培过程具有广适性。
Tissue culture is one of the important methods for the rapid propagation of bamboo plants.Based on the CNKI and WOS databases,in this article,bibliometric analysis and statistical methods were used to conduct an in-depth study of the number of publications,time,region,genera and species,types of tissue culture,and explant selection of bamboo tissue culture during 1960-2020.The results showed that the bamboo tissue culture researches were mainly conducted in developing countries,because of the distribution of bamboo species,population structure,and economic and technological development level,and research authors and institutions presented the characteristics of local gathering and global dispersion.The most studied bamboo species were Dendrocalamus,Bambusa and Phyllostachys species,this was related to their characteristics,uses and tissue culture techniques.Bamboo tissue culture types were mainly based on the following three kinds of methods,i.e.,bud propagation,embryo culture and callus regeneration plants.Disinfectant mercury chloride,ethanol and sodium hypochlorite solution,medium MS,3/4MS and 1/2MS,growth regulator BA,IBA and NAA,etc.had wide adaptability to bamboo tissue culture process.
作者
李雪梅
吕丹丹
张文根
陈天国
杨光耀
LI Xue-mei;LV Dan-dan;ZHANG Wen-gen;CHEN Tian-guo;YANG Guang-yao(Jiangxi Agricultural University,Jiangxi Province Key Laboratory of Bamboo Germplasm Resources and Utilization,Nanchang 330045,Jiangxi,China;Changzhou Agricultural Technology Extension Center,Changzhou 213000,Jiangsu,China)
出处
《竹子学报》
2020年第4期25-33,共9页
Journal of Bamboo Research
基金
江西省重点研发计划项目(20192BBF60015)。
