摘要
目的观察不同碘摄入水平对Wistar大鼠妊娠后甲状腺功能的影响,为孕期科学补碘及甲状腺功能筛查提供实验依据。方法选择断乳2周的SPF级雌性Wistar大鼠150只,饮用含碘化钾(KI)去离子水进行雌性Wistar大鼠碘营养干预,并按照随机数字表法将雌性Wistar大鼠分为5组[严重碘缺乏(SID)组、轻度碘缺乏(MID)组、对照(NI)组、轻度碘过量(MIE)组、严重碘过量(SIE)组,每组30只],5组大鼠的碘摄入量分别为0.0、1.5、5.5、70.0和350.0μg/d。建立动物模型并干预3个月,检测大鼠24 h尿碘含量并与NI组比较以判定模型是否构建成功。造模成功后,将受试雌性Wistar大鼠与雄性Wistar大鼠交配(雌雄比为2~3∶1)。每组妊娠大鼠约为15只,以与造模条件相同的剂量继续对大鼠干预21 d。将未受孕与妊娠大鼠麻醉取腹主动脉血,待分离血清后检测各组大鼠的血清甲状腺功能指标。结果5组大鼠尿碘含量比较差异有统计学意义(尿碘中位数分别为3.540、51.410、286.801、644.192、2368.701,H=94.791,P<0.01),不同碘营养水平大鼠造模成功。未受孕大鼠各组间促甲状腺激素(TSH)水平、抗甲状腺过氧化物酶抗体(TPOAb)和双抗体阳性率比较,差异均无统计学意义(P均>0.05);而游离甲状腺素(FT_(4))、游离三碘甲状腺原氨酸(FT_(3))水平及抗甲状腺球蛋白抗体(TgAb)阳性率组间比较,差异均有统计学意义(P均<0.05),SID组FT_(4)水平低于NI组(P<0.05),FT_(3)水平高于NI组(P<0.05);SIE组的TgAb阳性率高于NI组(P<0.05)。妊娠大鼠各组间TSH、FT_(4)、FT_(3)水平比较差异均无统计学意义(P均>0.05);而TgAb、TPOAb和双抗体阳性率组间比较,差异均有统计学意义(P均<0.05),MIE、SIE组TgAb阳性率均高于NI组(P均<0.05);MIE组TPOAb阳性率高于NI组(P<0.05),MID、MIE组双抗体阳性率均高于NI组(P均<0.05)。结论碘缺乏可导致未受孕大鼠甲状腺激素水平改变,而碘过量可引起未受孕和妊娠大鼠相关抗体阳性率升高。
Objective To investigate the effects of different levels of iodine intake on thyroid function in Wistar rats after pregnancy,and to provide experimental basis for scientific supplementation of iodine and thyroid function screening during pregnancy.Methods One hundred and fifty female SPF Wistar rats weaned for 2 weeks were selected.Female Wistar rats were intervened with iodine nutrition by drinking deionized water containing potassium iodide(KI).According to the random number table,female Wistar rats were divided into 5 groups[severe iodine deficiency(SID)group,mild iodine deficiency(MID)group,control group(NI),mild iodine excess(MIE)group,and severe iodine excess(SIE)group,30 rats per group].The iodine doses of the five groups were 0.0,1.5,5.5,70.0 and 350.0μg/d,respectively.The animal model was established and intervened for 3 months to detect the 24 h urinary iodine content of rats,and compared with the NI group to determine the success of the model or not.After successful modeling,the tested female Wistar rats were mated with male Wistar rats(female:male=2-3:1).There were about 15 pregnant rats in each group,and continued to intervene the pregnant rats for 21 d with the same doses as the modeling conditions.The abdominal aortic blood of non-pregnant and pregnant rats was taken.After serum separation,5 items[free thyroxine(FT_(4)),free triiodothyronine(FT_(3)),thyroid stimulating hormone(TSH),anti-thyroglobulin antibodies(TgAb),thyroid peroxidase antibody(TPOAb)]of serum thyroid function were detected in each group.Results There was significant difference in urinary iodine content between the five groups(the medians urinary iodine were 3.540,51.410,286.801,644.192 and 2368.701,respectively,H=94.791,P<0.01).Rats with different iodine nutrition levels were successfully established.There were no significant differences in TSH level,TPOAb and double antibodies positive rates of non-pregnant rats among groups(P>0.05).There were statistically significant differences in the levels of FT_(4),FT_(3) and the positive rate of TgAb among groups(P<0.05).The level of FT_(4) in SID group was lower than that in NI group(P<0.05).The level of FT_(3) in SID group was higher than that in NI group(P<0.05).The positive rate of TgAb in SIE group was higher than that in NI group(P<0.05).There were no significant differences in TSH,FT_(4) and FT_(3) levels of pregnant rats among groups(P>0.05).There were significant differences in the positive rates of TgAb,TPOAb and double antibodies among the groups(P<0.05).The positive rates of TgAb,TPOAb and double antibodies in MIE and SIE groups were higher than those in NI group(P<0.05).The positive rate of TPOAb in MIE group was higher than that in NI group(P<0.05),and the positive rates of double antibodies in MID and MIE groups were higher than that in NI group(P<0.05).Conclusions Iodine deficiency can lead to the change of thyroid hormone level in non pregnant rats,while iodine excess can increase the positive rate of related antibodies in non pregnant and pregnant rats.
作者
祝宇铭
黄佳
张荣
张伟
林勤
Zhu Yuming;Huang Jia;Zhang Rong;Zhang Wei;Lin Qin(Institute for Environmental Health and Endemic Disease Control,Xinjiang Center for Disease Control and Prevention,Urumqi 830002,China;Institute of Environmental and Health-related Product Safety,Chinese Center for Disease Control and Prevention,Beijing 100021,China)
出处
《中华地方病学杂志》
CAS
北大核心
2021年第9期689-693,共5页
Chinese Journal of Endemiology
基金
新疆维吾尔自治区卫生健康青年医学科技人才专项(WJWY-202016)。
关键词
甲状腺激素
碘营养
妊娠大鼠
Thyroid hormone
Iodine nutrition
Pregnant rats
