摘要
目的探讨甲状腺功能亢进症(简称甲亢)患者外周血单个核细胞微小RNA(miR)-206表达水平与Wnt/β-连环蛋白(β-catenin)通路中Wnt-1和β-catenin表达水平的相关性。方法采用前瞻性设计,选择2018年1月至2020年6月武威市人民医院收治的甲亢患者79例作为观察组,另选择2019年1月至2020年9月武威市人民医院健康体检者70例作为对照组。观察组使用抗甲状腺药物治疗,于治疗期口服他巴唑10~20 mg/d;血清甲状腺激素正常后减量,维持他巴唑5~10 mg/d,持续治疗12个月。采用实时荧光定量PCR(qRT-PCR)检测观察组治疗前后以及对照组外周血单个核细胞miR-206,Wnt-1、β-catenin mRNA表达水平。结果观察组治疗前外周血单个核细胞miR-206表达水平低于对照组(0.28±0.07比0.79±0.15),Wnt-1和β-catenin mRNA表达水平均高于对照组(6.75±1.39比2.23±0.65,5.83±1.24比3.21±0.86,P均<0.05)。观察组治疗后外周血单个核细胞miR-206表达水平(0.54±0.13)高于治疗前,Wnt-1和β-catenin mRNA表达水平(3.62±1.08、4.34±1.16)均低于治疗前(P均<0.05)。甲亢患者外周血单个核细胞miR-206表达水平与Wnt-1、β-catenin mRNA表达水平均呈负相关(r=-0.763、-0.798,P均<0.05)。结论甲亢患者外周血单个核细胞miR-206低表达,而Wnt-1和β-catenin mRNA高表达,miR-206表达水平与Wnt-1、β-catenin mRNA表达水平均呈负相关。
Objective To explore the correlation between the expression level of microRNA(miR)-206 and the expression levels of Wnt-1 and β-catenin in Wnt/β-catenin pathway in peripheral blood mononuclear cells of patients with hyperthyroidism.Methods Using a prospective design,79 patients with hyperthyroidism admitted to Wuwei City People's Hospital from January 2018 to June 2020 were selected as observation group,and 70 cases of healthy physical examination in Wuwei City People's Hospital from January 2019 to September 2020 were selected as control group.The observation group was treated with anti-thyroid drugs,and tambazole was orally taken 10-20 mg/d during the treatment period;after the serum thyroid hormone was normal,the dosage was reduced and maintained at 5-10 mg/d for 12 months.Quantitative real-time PCR(qRT-PCR)was used to detect the expression levels of miR-206,Wnt-1 and β-catenin mRNA in peripheral blood mononuclear cells in observation group before and after treatment and control group.Results The expression level of miR-206 in peripheral blood mononuclear cells of observation group before treatment was lower than that of control group(0.28±0.07 vs 0.79±0.15),and the expression levels of Wnt-1 and β-catenin mRNA were higher than those of control group(6.75±1.39 vs 2.23±0.65,5.83±1.24 vs 3.21±0.86,P<0.05).The expression level of miR-206(0.54±0.13)in peripheral blood mononuclear cells of observation group after treatment was higher than that before treatment,and the expression levels of Wnt-1 and β-catenin mRNA(3.62±1.08,4.34±1.16)were lower than those before treatment(P<0.05).The expression level of miR-206 in peripheral blood mononuclear cells of patients with hyperthyroidism was negatively correlated with the expression levels of Wnt-1 andβ-catenin mRNA(r=-0.763,-0.798,P<0.05).Conclusions The expression level of miR-206 in peripheral blood mononuclear cells of patients with hyperthyroidism is low,while the expression levels of Wnt-1 and β-catenin mRNA are high.The expression level of miR-206 is negatively correlated with the expression levels of Wnt-1 and β-catenin mRNA.
作者
王洪涛
梁育岑
秦文科
Wang Hongtao;Liang Yucen;Qin Wenke(Department of General Surgery,Wuwei City People's Hospital,Wuwei 733000,China)
出处
《中华地方病学杂志》
CAS
北大核心
2021年第9期694-698,共5页
Chinese Journal of Endemiology
