摘要
目的:利用试验方法探究SDF-1/CXCR4生物轴在胃癌淋巴转移中可能分子机制。方法:2017年6月-2020年10月间在医院普外科对胃癌根治术32例患者,在知情同意下采集胃组织标本,分为正常胃黏膜组织、癌旁组织、胃癌组织、淋巴结转移组织,对SDF-1、CXCR4、MMP-9、MMP-2表达行免疫组化法检测,进一步分析与胃癌临床病理特征的关系。结果:胃癌淋巴转移组织中SDF-1、CXCR4、MMP-2、MMP-9阳性表达率分别为93.75%、90.63%、93.75%、93.75%,高于正常胃黏膜组织、癌旁组织及胃癌组织,与肿瘤大小、TNM分期有着密切联系。结论:对于胃癌,SDF-1/CXCR4生物轴可能通过调节MMP-9、MMP-2表达促进淋巴转移,并且与肿瘤大小及TNM分期等临床病理特征关系密切,为临床判断胃癌治疗效果、预后提供参考。
Objective: To explore the possible molecular mechanism of SDF-1/CXCR4 biological axis in lymphatic metastasis of gastric cancer using experimental methods. Methods: Collected 32 patients undergoing radical gastric cancer surgery in the General Surgery Department of Inner Mongolia Autonomous Region Tumor Hospital from June 2017 to December 2018. Collected the specimens of gastric cancer tissue, paracancerous tissue, lymph node metastasis tissue and normal gastric mucosal tissue, and detect to detect the expression of SDF-1, CXCR4, MMP-9, MMP-2 in the tissues by immunohistochemical method, and analyze the relationship with the clinicopathological characteristics of gastric cancer. Results: The positive expression rates of SDF-1, CXCR4, MMP-2, and MMP-9 in lymphatic metastasis tissues were 93.75%, 90.63%,93.75%, 93.75%, respectively, which were higher than those in gastric cancer tissue, paracancerous tissue and normal gastric mucosal tissue, and are closely related to the size of gastric cancer tumors and TNM staging. Conclusion: For gastric cancer,the SDF-1/CXCR4 biological axis may promote lymphatic metastasis by regulating the expression of MMP-9 and MMP-2,and is closely related to the tumor size and TNM staging and other clinicopathological characteristics, to provide reference for which clinic judge gastric cancer’s treatment effect and prognosis.
作者
张锐
孔令惠
张志强
李永乐
ZHANG Rui;KONG Ling-hui;ZHANG Zhi-qing(Tumor Hospital of inner Mongolia Autonomous Region,Hohhot 010020 China)
出处
《疾病监测与控制》
2021年第3期177-181,共5页
Journal of Diseases Monitor and Control
基金
内蒙古医科大学科技百万工程(联合)项目(YKD2016KJBW(LH)025)
呼和浩特市科学技术协会内蒙古自治区肿瘤医院基金项目。
