摘要
目的考察桃红四物汤对去卵巢骨质疏松模型大鼠骨密度、骨形态学和骨载荷能力的影响,并探讨其可能的作用机制。方法SD大鼠随机分为对照组、模型组、仙灵骨葆胶囊(0.4 g/kg)组以及桃红四物汤低、高剂量(0.2、0.4 g/kg)组,每组8只。除对照组外,其余各组大鼠去除双侧卵巢建立骨质疏松模型。各给药组ig相应药物,连续3个月。采用三点弯曲实验检测各组大鼠股骨荷载能力;采用苏木素-伊红(HE)和番红固绿染色观察各组大鼠胫骨病理变化;采用微计算机断层扫描技术(micro computed tomography,Micro-CT)分析各组大鼠胫骨骨密度、骨小梁宽度和分离度等形态学参数;采用抗酒石酸酸性磷酸酶(Tartrate-resistant acid phosphatase,TRAP)染色检测各组大鼠胫骨破骨细胞数量;采用免疫组化法检测各组大鼠胫骨骨保护素(osteoprotegerin,OPG)和核因子-κB受体活化因子配体(receptor activator for nuclear factor-κB ligand,RANKL)蛋白表达情况。结果与模型组比较,桃红四物汤组大鼠股骨最大载荷、最大应力和弹性载荷均显著升高(P<0.05、0.01);胫骨骨丢失程度改善,骨小梁数量和连接增多;胫骨骨密度、骨小梁与骨组织面积比、骨小梁宽度和骨小梁数量显著升高(P<0.05、0.01),骨小梁分离度显著降低(P<0.01);胫骨破骨细胞数量减少,OPG表达增加,RANKL表达降低。结论桃红四物汤能够增加骨密度及荷载能力,促进成骨细胞功能,抑制破骨细胞功能亢进引起的骨吸收,从而发挥抗骨质疏松作用。
Objective To investigate the effect of Taohong Siwu Decoction(桃红四物汤)on bone mineral density,bone morphology and bone load capacity of ovariectomized osteoporosis model rats,and explore its possible mechanism.Methods SD rats were randomly divided into control group,model group,Xianling Gubao Capsule(0.4 g/kg)group,low-and high-dose Taohong Siwu Decoction(0.2,0.4 g/kg)groups,with eight rats in each group.Except for control group,rats in the other groups were removed bilateral ovaries to establish an osteoporosis model.Rats in each administration group were ig corresponding drugs for three consecutive months.Three-point bending test was used to detect the load capacity of femur of rats in each group;Hematoxylin-eosin(HE)and Safranin fast green staining were used to observe the pathological changes of tibia of rats in each group;Micro computed tomography(Micro-CT)was used to analyze the morphological parameters such as bone density,trabecular bone width and separation of tibia of rats in each group;Tartrate-resistant acid phosphatase(TRAP)staining was used to detect the number of tibia osteoclasts of rats in each group;Immunohistochemical method was used to detect the protein expression of osteoprotegerin(OPG)and receptor activator for nuclear factor-κB ligand(RANKL)of rats in each group.Results Compared with model group,maximum load,maximum stress and elastic load of femur of rats in Taohong Siwu Decoction group were significantly increased(P<0.05,0.01);Tibia bone loss was improved,the number of bone trabeculae and connection was increased;Tibia bone density,ratio of trabecular bone area to bone tissue area,trabecular bone width and number of trabecular bone were significantly increased(P<0.05,0.01),trabecular bone separation was significantly decreased(P<0.01);Number of tibial osteoclasts was decreased,OPG expression was increased,and RANKL expression was decreased.Conclusion Taohong Siwu Decoction can increase bone density and load capacity,promote osteoblast function,reduce bone resorption caused by osteoclast hyperfunction,and exert anti-osteoporosis effect.
作者
徐志红
陈磊垚
许立
张娴
XU Zhi-hong;CHEN Lei-yao;XU Li;ZHANG Xian(Nanjing Integrated Traditional Chinese and Western Medicine Hospital Affiliated to Nanjing University of Chinese Medicine,Nanjing 210014,China)
出处
《中草药》
CAS
CSCD
北大核心
2021年第18期5608-5614,共7页
Chinese Traditional and Herbal Drugs
基金
南京市医学科技发展项目(QYK11148)。
