摘要
目的探讨成纤维细胞焦亡在创面愈合中的作用及虎杖苷对其的影响。方法以5μg/ml脂多糖(LPS)刺激成纤维细胞建立实验模型,并随机分为4组:对照组细胞不接受任何处理;实验组细胞接受5μg/mlLPS处理;治疗组细胞接受5μg/mlLPS及50μmol/L虎杖苷处理;抑制剂组细胞接受5μg/mlLPS及100 nmol/LVX-765处理。四甲基偶氮唑蓝(MTT)法检测细胞增殖;试剂盒检测caspase-1活性;酶联免疫吸附试验(ELISA)检测培养基白细胞介素(IL)-1β、IL-18及Ⅲ型胶原蛋白含量。结果与对照组比较,实验组caspase-1活性及IL-1β、IL-18含量分别显著增加为(475.4±39.9)%、(254.9±19.4)pg/ml及(212.6±22.4)pg/ml,细胞增殖及Ⅲ型胶原蛋白含量分别显著下降为(72.5±3.4)%及(0.06±0.01)μg/L(均P<0.05);与实验组比较,治疗组caspase-1活性及IL-1β、IL-18含量分别显著下降为(292.1±28.5)%、(167.5±11.8)pg/ml及(165.5±19.9)pg/ml,细胞增殖及Ⅲ型胶原蛋白含量分别显著增加为(86.8±5.5)%及(0.10±0.02)μg/L(均P<0.05);与实验组比较,抑制剂组caspase-1活性及IL-1β、IL-18含量分别显著下降为(185.5±16.1)%、(127.4±16.5)pg/ml及(129.5±17.2)pg/ml,细胞增殖及Ⅲ型胶原蛋白含量分别显著增加为(83.1±7.7)%及(0.09±0.01)μg/L(均P<0.05)。结论抑制LPS诱导的成纤维细胞焦亡可以促进其增殖并分泌胶原蛋白,虎杖苷可能通过抑制成纤维细胞焦亡促进创面愈合。
Objective To investigate the effect of polydatin on the pyroptosis of fibroblasts in wound healing.Methods Fibroblasts were stimulated with 5μg/ml of lipopolysaccharide(LPS)to establish the experimental model,and were randomly divided into four groups as following:cells in the control group received none treatment;cells in the experimental group were treated with 5μg/ml of LPS;cells in the treatment group were treated with 5μg/ml of LPS and 50μmol/L of polydatin;cells in the inhibitor group were treated with 5μg/ml of LPS and 100 nmol/L of Belnacasan(VX-765).Cell proliferation was detected by 3-(4,5)-dimethylthiahiazo(-2)-3,5-diphenytetrazoliumromide(MTT)assay.Caspase-1 activity was detected by the commercial kit.The contents of interleukin(IL)-1β,IL-18,and type Ⅲ collagen in the culture medium were determined by enzyme linked immunosorbent assay(ELISA).Results Compared with those in the control group,the caspase-1 activity and IL-1β and IL-18 contents in the experimental group significantly increased to(475.4±39.9)%,(254.9±19.4)pg/ml,and(212.6±22.4)pg/ml,respectively,and the cell proliferation and type Ⅲ collagen content significantly decreased to(72.5±3.4)%and(0.06±0.01)μg/L(all P<0.05).Compared with those in the experimental group,the caspase-1 activity and IL-1β and IL-18 contents in the treatment group decreased significantly to(292.1±28.5)%,(167.5±11.8)pg/ml,and(165.5±19.9)pg/ml,respectively,and the cell proliferation and type Ⅲ collagen content significantly increased to(86.8±5.5)%and(0.10±0.02)μg/L(all P<0.05).Compared with those in the experimental group,the caspase-1 activity and IL-1β and IL-18 contents in the inhibitor group significantly decreased to(185.5±16.1)%,(127.4±16.5)pg/ml,and(129.5±17.2)pg/ml,respectively,and the cell proliferation and type Ⅲ collagen content significantly increased to(83.1±7.7)%and(0.09±0.01)μg/L(all P<0.05).Conclusion Inhibition of LPS-induced pyroptosis promotes the proliferation and secretion of collagen of fibroblasts.Polydatin may promote the wound healing by inhibiting the pyroptosis of fibroblasts.
作者
陈白烨
陈敏健
王学明
宋湧
汪怡
袁晓野
Chen Baiye;Chen Minjian;Wang Xueming;Song Yong;Wang Yi;Wang Xiaoye(Department of Plastic Surgery,Fujian Provincial Maternity and Children's Hospital,Fuzhou 350000,China)
出处
《国际医药卫生导报》
2021年第20期3186-3188,共3页
International Medicine and Health Guidance News
