摘要
为评价中药复方提取物治疗兔肠道球虫病的效果,试验将60只轻度感染肠道球虫的新西兰白兔随机分为6组,分别为感染不用药组、中药高剂量组、中药中剂量组、中药低剂量组、地克珠利组和氯苯胍组,均人工接种1×105个孢子化卵囊(大型艾美耳球虫、中型艾美耳球虫、黄艾美耳球虫和小型艾美耳球虫孢子化卵囊的混合物)。接种后第3天,前4组分别每天灌服生理盐水及1,0.5,0.2 g/mL中药提取物各8 mL;地克珠利组每天在饮水中按0.3 mL/L添加5%地克珠利溶液;氯苯胍组每天在饲料中按600 mg/kg添加氯苯胍,连用7 d,随后停止用药。每天观察并记录兔的临床症状,间隔7 d采集所有兔粪便,检测每克粪便卵囊数(OPG),记录每只兔增重和死亡情况,计算存活率,并对死亡兔进行剖检。结果表明:中药高剂量组、地克珠利组和氯苯胍组兔存活率均为100%,3组兔治疗7,14,21后OPG均差异不显著(P>0.05),中药高剂量组和氯苯胍组兔试验期间增重差异不显著(P>0.05),地克珠利组在试验期间平均增重显著高于氯苯胍组和中药高剂量组(P<0.05)。中药中剂量组兔存活率为90%,其OPG与氯苯胍组、地克珠利组和中药高剂量组均差异不显著(P>0.05),试验期间平均增重显著低于地克珠利组(P<0.05)。中药低剂量组兔存活率为70%,其给药后第14,21天OPG、试验期间平均增重均显著低于其他用药组(P<0.05)。说明中药复方制剂治疗兔肠道球虫病存在量效关系,高剂量治疗效果最佳。
In order to evaluate the effect of Chinese herbal compund extracts on rabbit intestinal coccidiosis,in this experiment 60 New Zealand white rabbits with mild intestinal coccidiosis infection were randomly divided into six groups,namely the infection without medication group,the high-dose Chinese herbal group,the medium-dose Chinese herbal group,the low-dose Chinese herbal group,the diclazuril group and the chloroguanide group,which were all artificially inoculated with 1×105 sporulated oocysts(Eimeria magna,Eimeria media,Eimeria flavescens and Eimeria exigua).On the 3rd day after inoculation,the first four groups were administrated via the gastrointestinal tract respectively with 8 mL of physiological saline,1,0.5,0.2 g/mL Chinese herbal extracts every day;the diclazuril group were given drinking water added 0.3 mL/L diclazuril solution of 5%every day;the chloroguanide group were fed with diet added 600 mg/kg chloroguanide every day.The medication was for seven days,and then stopped.The clinical symptoms of rabbits were observed and recorded every day;all rabbit feces were collected at an interval of seven days,and the number of oocysts per gram of feces(OPG)were detected;the weight gain and death of each rabbit were recorded,the survival rate was calculated,and an autopsy on the dead rabbits was performed.The results showed that the survival rates of rabbits were the same 100%in high-dose Chinese herbal group,the diclazuril group and the chloroguanide group;there was no significant difference in OPG between the three groups of rabbits on the 7th,14th,and 21th day post-treatment(P>0.05).There was no significant difference in rabbit weight gains between the high-dose Chinese herbal group and the chloroguanide group(P>0.05).The average weight gain in the diclazuril group during the trial period was significantly higher than those in chloroguanide group and high-dose Chinese herbal group(P<0.05).The survival rate of rabbits in medium-dose Chinese herbal group was 90%;its OPG had no significant difference compared with those in chloroguanide group,diclazuril group,high-dose Chinese herbal group(P>0.05);and its average weight gain was significantly lower than that in the diclazuril group.The survival rate of rabbits in low-dose Chinese herbal group was 70%;its OPG and weight gain were all significantly lower than those in other treatment groups on the 14th,and 21th day post-treatment(P<0.05).The results suggested that there was a dose-effect relationship in the treatment of Chinese herbal compound medicine on rabbit intestinal coccidiosis,and the higher dose treatment had the better effect.
作者
叶勇刚
于吉锋
魏勇
叶健强
谢晶
张先惠
钟光旭
吴学婧
康润敏
YE Yonggang;YU Jifeng;WEI Yong;YE Jianqiang;XIE Jing;ZHANG Xianhui;ZHONG Guangxu;WU Xuejing;KANG Runmin(Key Laboratory of Animal Genetics and Breeding of Sichuan Province,Sichuan Animal Science Academy,Chengdu 610066,China;Leibo County Bureau of Agriculture and Rural Affairs,Leibo 616550,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2021年第18期115-118,154,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
四川省应用基础项目(2019YJ0562,2019YJ0563)
四川省财政运行专项(SASA2020CZYX006)。
关键词
中药复方提取物
兔
卵囊
肠道球虫病
治疗
效果
Chinese herbal compound extracts
rabbit
oocyst
intestinal coccidiosis
treatment
effect
