摘要
本研究旨在阐明鸭疫里默氏杆菌RA-LZ01株GE296_RS01450基因介导其对重金属离子产生耐受性的作用。构建RA-LZ01株的GE296_RS01450基因缺失株Δ1450和回复株cΔ1450,测定菌株的生长曲线、抗菌素对菌株的最小抑菌浓度(MIC)、菌株对重金属离子的耐受程度以及GE296_RS01450基因在特异性重金属离子刺激下的转录水平。结果显示,与亲本株相比,缺失株的生长能力以及11类抗菌素对缺失株的MIC值均无明显变化;与亲本株和回复株相比,缺失株对重金属离子Ni、Mn和Co的敏感性显著上升,并且在Ni、Mn刺激下,GE296_RS01450基因的转录水平显著上调。上述结果表明,GE296_RS01450基因不参与亲本株的生长及菌株对抗菌素的耐药性,可介导菌株对Ni、Mn和Co产生耐受性。GE296_RS01450基因属于GE296_RS01445-GE296_RS01450-GE296_RS01455 RND外排泵,编码外膜蛋白,我们将其命名为RopM。
This study was conducted to clarify the role of GE296_RS01450 gene of Riemerella anatipestifer strain RA-LZ01 in mediating its tolerance to heavy metal ions.We constructed GE296_RS01450 gene deletion mutantΔ1450 and complemented strain cΔ1450.The growth curves of these strains,minimum inhibitory concentration(MIC)of antibiotics against them and their tolerance to heavy metal ions were determined,and the transcription levels of GE296_RS01450 gene of these strains treated with specific heavy metal ions were detected.The results showed that the growth ability of the deletion mutant and the MIC values of 11 classes of antibiotics to deletion mutant did not change significantly by comparison with the parent strain.By compared with parent strain and complemented strain,the sensitivity of deletion mutant to Ni,Mn and Co increased significantly.The transcription levels of GE296_RS01450 gene of parent strain treated with Ni and Mn were significantly up-regulated.The above results indicate that the GE296_RS01450 gene does not involve in the growth of the parent strain and the strain’s resistance to antibiotics.However,the gene mediates the parent strain’s tolerance to Ni,Mn and Co.GE296_RS01450 gene belongs to the GE296_RS01445-GE296_RS01450-GE296_RS01455 RND efflux pump and encodes the outer membrane protein.This protein is named RopM.
作者
秦明星
宫晓炜
陈启伟
王燕萍
权衡
朱雨佳
郑福英
蔺国珍
QIN Mingxing;GONG Xiaowei;CHEN Qiwei;WANG Yanping;QUAN Heng;ZHU Yujia;ZHENG Fuying;LIN Guozhen(College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China;State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2021年第10期2864-2873,共10页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
西北民族大学预防兽医学创新团队项目(110130143)
甘肃省自然科学基金项目(21 JR1RA203)
中央高校基本科研业务费专项资金(31920180007)
中国农业科学院中央级公益性科研院所基本科研业务费专项(1610312020001)。
