摘要
目的探讨自身免疫性肝病和病毒性肝炎患者血清中抗肝抗体检测的临床意义。方法纳入我院收治的110例自身免疫性肝病(ALDS)和350例病毒性肝炎患者的血清标本,入选时间为2016年3月至2019年3月;选取同期我院40名健康体检者作为健康对照组。采用免疫印迹法检测所有研究对象的血清中的抗肝自身抗体,并测定其肝功能。结果在抗肝抗体检测中,ALDS组的中线粒体抗体(AMA)、抗平滑肌抗体(SMA)、抗可溶性肝抗原/肝胰抗原(SLA/LP)、线粒体抗体(AMA)、抗肝肾微粒体I型(LKM-1)、抗线粒体抗体亚型-丙酮酸脱氢酸合物(AMA-2)以及抗肝细胞溶质抗原I型抗体(LC-1)的阳性检出率均明显多于病毒性肝炎组,且3组间对比差异有统计学意义(P<0.05)。在肝病自身抗体检测中,自身免疫性肝炎(AIH)组的SMA阳性率明显高于原发性胆汁肝硬化(PBC)组,但其AMA阳性率则显著低于PBC组(P<0.05)。在肝功能检测中,ALDS组的碱性磷酸酶(ALP)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)及谷氨酰转肽酶(GGT)等指标均明显高于病毒性肝病组及健康对照组,差异有统计学意义(P<0.05),但免疫球蛋白(IgG)与病毒性肝病组及健康对照组相比较,差异无统计学意义(P>0.05)。结论检测ALDS与病毒性肝病患者自身抗体对疾病的诊断与鉴别有重要意义,可提高诊断的准确性,在临床有积极的意义。
Objective To investigate the clinical significance of anti-liver antibody detection in serum of patients with autoimmune liver disease and viral hepatitis.Methods Serum samples from 110 patients with autoimmune liver disease(ALDS)and 350 patients with viral hepatitis admitted to our hospital were included in this study.The time span was from March 2016 to March 2019,40 cases of healthy physical examinees in our hospital were selected as healthy control group during the same period.Anti-hepatic autoantibodies in serum of all subjects were detected by western blotting and liver function was measured.Results In anti liver antibody detection,ALDS group of mitochondrial antibody(AMA),smooth muscle antibodies(SMA)resistance,resistance to soluble liver antigen/pancreas antigen(SLA/LP),mitochondrial antibody(AMA),liver and kidney microsomal type I(LKM-1),antimitochondrial antibody subtypes of pyruvate dehydrogenase acid compound(AMA-2)and anti liver cytosol antigen antibody of type I(LC-1)the positive detection rate of group were significantly greater than viral hepatitis,and compared between three groups of data are statistically significant(P<0.05).In the autoantibody test of liver disease,the SMA positive rate in the autoimmune hepatitis(AIH)group was significantly higher than that in the primary biliary cirrhosis(PBC)group,but the AMA positive rate was significantly lower than that in the PBC group(P<0.05).In liver function tests,ALDS,alkaline phosphatase(ALP),aspartate aminotransferase(AST),alanine aminotransferase(ALT)and gamma glutamyl transpeptidase(GGT),liver index were significantly greater than viral liver disease group and health control group,the difference was statistically significant(P<0.05),but its immunoglobulin(IgG)and viral liver disease group and health control group there was no statistically significant difference in comparison(P>0.05).Conclusion The detection of autoantibodies from patients with ALDS and viral liver disease is of great significance in the diagnosis and differentiation of diseases,and can improve the accuracy of diagnosis.
作者
王娜
刘淼
花艳艳
WANG Na;LIU Miao;HUA Yanyan(Dalian Public Health Clinical Center(Dalian Sixth People's Hospital),Dalian 116031,China)
出处
《中国医药指南》
2021年第30期55-57,共3页
Guide of China Medicine
关键词
自身免疫性肝病
病毒性肝炎
血清
抗肝抗体
Autoimmune liver disease
Viral hepatitis
Serum
Anti-liver antibodies
