摘要
为了揭示SiCCT基因可能的功能效应,并进一步研究该基因在光温互作调控谷子开花过程中所起的作用。以光周期敏感谷子品种黄毛谷为材料,利用荧光定量PCR技术分析了SiCCT基因在长日照高温(LD,27℃)、长日照低温(LD,22℃)、短日照高温(SD,27℃)、短日照低温(SD,22℃)4个光温组合条件下的昼夜表达规律,并在连续2 a调查10个主要农艺性状的基础上,开展了基于候选基因的关联分析。结果表明,光照期SiCCT基因表达不受温度影响,长日照条件表达水平整体高于短日照,而黑暗期表达受温度影响,特别是短日照条件下SiCCT基因在低温环境的表达水平明显高于高温环境。基于候选基因关联分析发现,SiCCT基因中共检测到11个多态性位点与8个主要农艺性状显著关联(P<0.05),其中SNP-10在河南洛阳、吉林吉林与抽穗期、叶片数和穗粒质量显著关联;SNP-100和SNP-104在河南洛阳同时都关联到株高、叶片数和穗长3个性状;SNP-51连续2 a在海南乐东、河南洛阳、吉林吉林都检测到,但关联到的性状不同,在海南乐东与穗粗关联,在河南洛阳与穗码数关联,在吉林吉林与叶片数关联。SiCCT基因表达受光周期调控,温度也影响其在短日照条件黑暗期的表达;SiCCT基因具有多效性,但其作用受光周期环境影响。
In order to reveal the possible functional effects of SiCCT gene,and provide foundation for further research of the function of SiCCT gene in photo-thermal interaction-controlled flowering process of foxtail millet,the photoperiod sensitive millet variety Huangmaogu was used as experimental material to analyze the diurnal expression patterns of SiCCT gene under long-day and high temperature(LD,27℃),long-day and low temperature(LD,22℃),short-day and high temperature(SD,27℃),short-day and low temperature(SD,22℃),totally four photo-thermal combinational conditions by fluorescence quantitative PCR.Furthermore,based on the survey data of ten main agronomic traits for two consecutive years,candidate gene-based association analysis was performed.The results showed that the expression of SiCCT gene was not affected by temperature,and the expression level of SiCCT gene under long-day was wholly higher than that under short-day at light period.While at darkness period,the expression of SiCCT gene was affected by temperature,especially under short-day,the expression level of SiCCT gene at low temperature was obviously higher than that at high temperature.Based on the association analysis of candidate gene,it was found that 11 polymorphic loci detected in SiCCT gene were significantly associated with 8 major agronomic traits(P<0.05),among which,SNP-10 was associated with heading stage,leaf number and grain weight per panicle at Luoyang,Henan and Jilin,Jilin,SNP-100 and SNP-104 were associated with plant height,leaf number and panicle length at Luoyang.SNP-51 was detected at Ledong of Hainan,Luoyang and Jilin for two consecutive years,but the related traits were different,which were ear diameter,branch number per panicle and leaf number at Ledong,Luoyang and Jilin,respectively.The expression of SiCCT gene was controlled by photoperiod,and temperature also affected its expression at darkness period under short-day.SiCCT gene possessed pleiotropic effects,but its function was regulated by photoperiod condition.
作者
王振山
袁玺垒
闫留延
杨朋娟
桑璐曼
贾小平
白俊艳
WANG Zhenshan;YUAN Xilei;YAN Liuyan;YANG Pengjuan;SANG Luman;JIA Xiaoping;BAI Junyan(College of Agriculture,Henan University of Science and Technology,Luoyang 471023,China;College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471023,China)
出处
《华北农学报》
CSCD
北大核心
2021年第5期43-49,共7页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31471569)。
关键词
谷子
光周期
温度
SiCCT基因
农艺性状
关联分析
Setaria italica
Photoperiod
Temperature
SiCCT gene
Agronomic trait
Association analysis
