摘要
为了解不同普通栽培品种枇杷中FCA基因对花发育的影响,延长枇杷的销售时间,以花期相差一个月且开花数量差异较大的‘解放钟’及西班牙枇杷品种‘爱沙拉’两个枇杷品种的芽、花苞和花为材料,提取RNA并克隆获得‘解放钟’和‘爱沙拉’两个枇杷品种的EjFCA基因,将获得的EjFCA基因进行测序以及生物信息学分析.结果显示,‘解放钟’和‘爱沙拉’中EjFCA基因的CDS序列分别为1125 bp和1137 bp、分别编码374和378个氨基酸,二者的相似度高达98.15%.利用生物信息学软件分析表明两个枇杷EjFCA基因均含有1个RRM结构域和1个WW结构域,保守性较高,为GATA类转录因子;亚细胞定位预测显示EjFCA蛋白位于细胞核,与苹果和白梨的亲缘关系最近.对EjFCA在上述两个枇杷品种的表达分析显示EjFCA基因在两个枇杷品种的芽、花苞和花中均有表达,在‘解放钟’中呈"先上升后下降"的趋势,花苞时期表达量最高,在盛花期略有下降,但在‘爱沙拉’枇杷的3个花期中表达量变化不大,且始终低于‘解放钟’.本研究表明调控‘爱沙拉’枇杷花期的主要基因不是EjFCA,但其花芽的形成可能受EjFCA调控.上述结果可为进一步研究蔷薇科植物的成花机理以及枇杷的遗传改良育种奠定基础.
The purpose of this study was to understand the effect of FCA on flower development in two different cultivars of loquat.Buds,alabastrums,and flowers of the loquat varieties‘Jiefangzhong’and‘Aixaza’were used as study materials.EjFCA from the two loquats was cloned using RNA extraction and was sequenced and analyzed via bioinformatics.The coding sequences of EjFCA in‘Jiefangzhong’and‘Aixaza’were 1125 bp and 1137 bp,encoding 374 and 378 amino acids,respectively.The homology between FCA of the two loquat cultivars was 98.15%.Bioinformatics analysis showed that the two loquat EjFCA contained one RRM domain and one WW domain,which were highly conserved.The results predicted by PlantTFDB indicated that EjFCA is a GATA transcription factor.The results predicted by subcellular localization showed that the EjFCA protein was located in the nucleus and microsome and had a close genetic relationship with apple and pear.EjFCA were expressed in buds,alabastrums,and flowers of‘Jiefangzhong’and‘Aixaza’.The expression of EjFCA was the highest during the alabastrum period and decreased slightly at the full flowering stage.However,the expression of EjFCA in‘Aixaza’changed little throughout the three flowering stages and was always lower than that in‘Jiefangzhong’.Although EjFCA was not the primary gene involved in the regulation of the flowering period of‘Aixaza’,it may regulate the formation of flower buds.
作者
吴毕莎
范金连
林授锴
许歆
马世伟
吴锦程
WU Bisha;FAN Jinlian;LIN Shoukai;XU Xin;MA Shiwei;WU Jincheng(College of Environmental and Biological Engineering,Putian University,Putian 351100,China;Key laboratory of Loquat Germplasm Innovation and Utilization(Putian University)of Fujian Province University,Putian 351100,China;Putian Customs District P.R.China,Putian 351100,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2021年第3期768-775,共8页
Chinese Journal of Applied and Environmental Biology
基金
福建省中青年教师教育科研项目(JA15454)资助。
