摘要
以采集自不同地区的14份自然发酵豆酱为试材,对其中的嗜盐四联球菌(Tetragenococcus halophilus)进行分离鉴定及益生特性研究,同时进一步探讨优良菌株的最佳生长条件,并通过单因素试验及响应面试验对其增殖培养基进行优化。结果表明,分离鉴定得到10株嗜盐四联球菌。其中,嗜盐四联球菌YSJ-5具有良好的耐酸、耐胆盐特性及抑菌特性,其最适培养条件为接种量3%、NaCl含量5%、培养温度33℃、初始pH值8.0。在此最适培养条件下,最适增殖培养基成分为葡萄糖2.70%,蛋白胨2.36%,黄豆浆4.41%,嗜盐四联球菌YSJ-5的活菌数为2.20×109CFU/mL。
Tetraphylococcus halophilus was isolated and identified from fourteen naturally fermented soybean paste collected from different areas, and its probiotic properties were studied. Meanwhile, the optimal growth conditions of the superior strain were further investigated, and the proliferation medium was optimized by single factor test and response surface test. The results showed that 10 strains of T. halophilus were isolated and identified. Among them,T. halophilus YSJ-5 had good acid tolerance, bile salt tolerance and bacteriostatic properties. The optimal culture conditions were inoculum 3%, salt concentration 5%, culture temperature 33 ℃, initial pH 8.0. Under the optimal culture conditions, the optimal proliferation medium components were glucose 2.70%, peptone 2.36% and soybean milk 4.41%, and the viable count of T. halophilus YSJ-5 was 2.20×109 CFU/ml.
作者
姜锦惠
赵悦
李盈烁
魏丽丽
赵越
杨雪萌
潘国杨
乌日娜
JIANG Jinhui;ZHAO Yue;LI Yingshuo;WEI Lili;ZHAO Yue;YANG Xuemeng;PAN Guoyang;WU Rina(Shenyang Key Laboratory of Microbial Fermentation Technology Innovation,Liaoning Food Fermentation Technology Engineering Research Center,College of Food Science,Shenyang Agricultural University,Shenyang 110866,China)
出处
《中国酿造》
CAS
北大核心
2021年第10期36-42,共7页
China Brewing
基金
国家自然科学基金项目(31972047)
辽宁省“兴辽英才计划”青年拔尖人才项目(XLYC1807040)
辽宁省自然科学基金地区联合基金项目(2020-MZLH-34)
辽宁省教育厅科学研究项目(LSNJC201902)
沈阳市中青年科技创新领军人才项目(RC200495)。
关键词
自然发酵豆酱
嗜盐四联球菌
分离筛选
鉴定
培养基优化
natural fermented soybean paste
Tetraphylococcus halophilus
isolation and screening
identification
medium optimization
