摘要
分别使用加水、加入酵母菌和加入复合菌3种方式对新鲜采集的油樟叶进行预处理,提取预处理后的油樟叶的油樟精油,并利用GC-MS鉴定油樟精油的化学成分;然后利用组织透明法对油樟叶中油细胞进行形态观察,统计油细胞密度;最后通过基因测序,表征油樟叶内生菌群落结构,分析内生菌群落与生物预处理的相关性。研究结果表明:酵母菌预处理和复合菌预处理使油樟精油提取得率分别提高33.6%和49.8%,并且使主成分1,8-桉叶素的相对含量略有降低,β-蒎烯、α-松油醇和γ-松油烯等次要成分略有提高;水预处理、酵母菌预处理和复合菌预处理后油樟叶油细胞密度分别为(37±2)、(27±2)和(25±5)个/mm^(2),低于新鲜油樟叶的油细胞密度,(37±3个)/mm^(2);基于距离冗余分析(dbRDA)和相关性热图分析预处理方式对油樟叶的内生菌群落结构影响较大,其中γ-变形菌、α-变形菌、蓝细菌和酸杆菌为受影响最大的菌群,3种预处理方式对内生菌群落结构影响的程度从大到小顺序为复合菌预处理>酵母菌预处理=水预处理;复合菌种的加入与多种核心内生菌种显著相关,尤其对浮霉菌、酸杆菌和梭状芽孢杆菌丰度的影响最为显著。
The leaves of Cinnamomum longepaniculatum(Gamble)N.Chao ex H.W.Li.were pretreated by adding water,yeast and complex bacteria respectively,and the essential oil of C.longepaniculatum leaves was extracted after pretreatment.The GC-MS was used to characterize the chemical composition of essential oil.The tissue transparency method was used to observe the morphology of the oil cells after pretreatment,and calculate the density of oil cells;finally,through gene sequencing,characterize the endophytic community structure of C.longepaniculatum leaves and analyze the correlation between the endophytic bacteria community and biological pretreatment.The results showed that yeast pretreatment and complex bacteria pretreatment increased the extraction yield of essential oil by 33.6%and 49.8%,respectively,and slightly reduced the relative content of 1,8-cineole,the minor components such asα-terpineol,β-pinene andγ-terpinene were slightly increased;the oil cell density of C.longepaniculatum leaves after pretreatment with water,yeast,and complex bacteria were(37±2),(27±2)and(25±5)per 1 mm^(2),which were less than that of fresh leaves,(37±3)per 1 mm^(2);Based on distance-based redundancy analysis(dbRDA)and correlation heatmap analysis,it is found that pretreatment methods havd a greater impact on the endophytic community structure of camphor leaves.γ-Proteobacteria,α-proteobacteria,cyanobacteria and acidobacteria were the most affected species in class level.The order of the degree of influence was complex bacteria pretreatment>yeast pretreatment=water pretreatment.The addition of complex bacteria was significantly related to a variety of endophytes,especially the abundance of planktomycetes,acid bacilli and bacillus.
作者
程贤
毕良武
李胜男
陈玉湘
赵振东
莫开林
CHENG Xian;BI Liangwu;LI Shengnan;CHEN Yuxiang;ZHAO Zhendong;MO Kailin(Institute of Chemical Industry of Forest Products,CAF,National Engineering Lab.for Biomass Chemical Utilization,Key Lab.of Chemical Engineering of Forest Products,National Forestry and Grassland Administration,Key Lab.of Biomass Energy and Material,Jiangsu Province,Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing 210042,China;Sichuan Academy of Forestry Sciences,Chengdu 610081,China)
出处
《林产化学与工业》
CAS
CSCD
北大核心
2021年第5期51-57,共7页
Chemistry and Industry of Forest Products
基金
四川省院省校合作项目(2019YFSY0002)
中国林科院林产化学工业研究所研究团队建设创新工程项目(LHSXKQ2)。
关键词
油樟
精油
油细胞
内生菌
Cinnamomum longepaniculatum
essential oil
oil cells
endophytic microbe
