摘要
目的:探讨川芎嗪(tetramethylpyrazine,TMP)对人肺癌A549细胞生长侵袭的干预作用与活性氧(reactive oxygen species,ROS)及其调控蛋白表达水平的相关性。方法:采用CCK-8法检测川芎嗪对A549细胞生长的影响,采用Transwell小室实验检测川芎嗪对A549细胞侵袭能力的影响,采用DCFH-DA荧光探针检测川芎嗪对A549细胞内ROS水平的影响,Western-blot法检测川芎嗪对HIF-1α、VEGF-C、MMP-9蛋白表达水平的影响。结果:川芎嗪呈浓度依赖性抑制肺癌A549细胞增殖和侵袭能力(P<0.05),同时减少细胞内ROS生成(P<0.05),抑制HIF-1α、VEGF-C、MMP-9蛋白表达(P<0.05)。结论:川芎嗪可降低人肺癌A549细胞ROS水平,减轻氧化应激损伤,从而抑制肿瘤细胞的增殖及侵袭。
Objective:To investigate the correlation between the intervention of TMP on the growth and invasion of A549 cells and the expression of reactive oxygen species(ROS)with regulated proteins level.Methods:To detect cell proliferation inhibition rate of A549 cells interfered with TMP in CCK-8.Transwell assay was used to determine the inhibitory effects of TMP on cell invasion.To observe intracellular ROS by fluorescence probe and expression of HIF-1α,VEGF-C,MMP-9 by Western-blot.Results:TMP inhibited A549 cells proliferation and invasion on dose(P<0.05).And the ROS production was also reduced with the expression of HIF-1α,VEGF-C,MMP-9 decreased(P<0.05).Conclusion:TMP can reduce the ROS level and oxidative stress injury of lung adenocarcinoma A549 cells,thus inhibit the invasion and proliferation of A549 cells.
作者
庞皓玥
胡凯文
孙满强
周天
王静
PANG Haoyue;HU Kaiwen;SUN Manqiang;ZHOU Tian;WANG Jing(Beijing University of ChineseMedicine,Beijing 100029,China;DongfangHospital,Beijing University of ChineseMedicine,Beijing 100078,China)
出处
《现代肿瘤医学》
CAS
北大核心
2021年第21期3695-3699,共5页
Journal of Modern Oncology
基金
全国中医药创新人才培养(编号:040101003063)
北京中医药大学基本科研业务费专项资金(编号:2018-JYBZZ-JS101)
北京中医药大学东方医院“1166”人才工程(编号:030903010335)。
