摘要
环境DNA(eDNA)是指环境中所有被发现的生物DNA的混合。eDNA技术可以通过对提取的环境样品进行DNA测序,反映生态系统中的群落或物种组成信息。相较于传统的基于形态学鉴定的生物监测方法,eDNA技术具有简单高效、灵敏度高、环境友好等优势,是未来水生态环境研究的重要手段之一。本文对eDNA技术的操作流程、在水生态环境研究中的应用及技术局限性进行了综述,并对eDNA技术的发展趋势进行了展望。
Environmental DNA(eDNA)refers to the DNA of all organisms found in the environment.eDNA can be helpful in determining community or species composition information in the ecosystem by analyzing DNA fragments directly from different environmental samples using sequencing techniques.Compared to traditional biological monitoring methods based on morphological identification,eDNA has the advantages of simplicity,high effectiveness,high sensitivity,and environmental friendliness.It is an important avenue for future research in aquatic ecosystems.Here,we review the operational flow of eDNA technology,its application in aquatic ecosystems,and existing problems,as well as prospective development trends of eDNA technology.
作者
赵彦伟
陈家琪
董丽
麻晓梅
白洁
田凯
ZHAO Yanwei;CHEN Jiaqi;DONG Li;MA Xiaomei;BAI Jie;TIAN Kai(State Key Laboratory of Water Environmental Simulation,School of Environment,Beijing Normal University,Beijing 100875,China)
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2021年第10期2057-2065,共9页
Journal of Agro-Environment Science
基金
国家自然科学基金项目(52070020)
水体污染控制与治理科技重大专项(2018ZX07110001)。
关键词
环境DNA
水生态系统
生物多样性
生态监测
应用局限
environmental DNA
aquatic ecosystem
biodiversity
ecological monitoring
application limitations
