摘要
建立一种同时测定糕点中乙二醇、1,2-丙二醇含量的方法,采用DB-WAX色谱柱(30 m×320μm×0.25μm),柱流速为1.0 mL/min,进样口为230℃,分流比为10∶1,检测器为氢火焰离子化检测器,检测器温度为240℃,空气流速350 mL/min,氢气流速40 mL/min,氮气流速25 mL/min,进样量1μL。结果表明,乙二醇、1,2-丙二醇分别在990~49500 ng/mL(r^(2)=0.9998)、980~49000 ng/mL(r^(2)=0.9997)范围内线性关系良好;在混合标准溶液75、100、200μg 3个添加水平下,乙二醇回收率为93.22%~101.10%,RSD为2.38%(n=9),1,2-丙二醇回收率为95.15%~99.97%,RSD为1.68%(n=9);乙二醇和1,2-丙二醇的定量限分别为6.53、6.49 mg/kg。该方法具有操作简便、重复性好、灵敏度好、所用试剂毒性小等特点,为糕点中乙二醇、1,2-丙二醇的含量检测提供参考。
Establish a method for the simultaneous determination of ethylene glycol and 1,2-propanediol in pastry,using DB-WAX column(30 m×320μm×0.25μm),column flow rate of 1.0 mL/min,sample inlet of 230℃,split ratio of 10∶1,detector was hydrogen flame ionization detector,detector temperature was 240℃,air flow speed was 350 mL/min,hydrogen flow rate was 40 mL/min,nitro⁃gen flow rate was 25 mL/min,injection volume was 1μL.The results showed that the linear relationship between ethylene glycol and 1,2-propanediol have good linearity in the range of 990~49500 ng/mL(r^(2)=0.9998)and 980~49000 ng/mL(r^(2)=0.9997),respective⁃ly.The recovery of ethylene glycol was 93.22%~101.10%,RSD was 2.38%(n=9),1,2-propanediol was 95.15%~99.97%,RSD was 1.68%(n=9)at the three levels of 75,100 and 200μg.The quantitative limits of ethylene glycol and 1,2-propanediol were 6.53 mg/kg and 6.49 mg/kg respectively.The method has the characteristics of simple operation,good repeatability,good sensitivity and small tox⁃icity of the reagents used.It can provide a reference for the content detection of ethylene glycol and 1,2-propanediol in pastry.
作者
丘一仙
陈婷婷
陈宇
唐明华
覃旭泉
QIU Yi-xian;CHEN Ting-ting;CHEN Yu;TANG Ming-hua;QIN Xu-quan(Yulin Center for Food and Drug Control,Yulin 537000,Guangxi,China;Rong County People's Hospital,Rongxian 537599,Guangxi,China)
出处
《湖北农业科学》
2021年第20期145-148,共4页
Hubei Agricultural Sciences
基金
玉林市科学研究与技术开发计划项目(玉市科201934034)。
关键词
糕点
气相色谱法
乙二醇
1
2-丙二醇
pastry
gas chromatography
ethylene glycol
1,2-propanediol
