摘要
目的:16S rDNA高通量测序观察1,25(OH)_(2)D_(3)对实验性自身免疫性神经炎肠道菌群的影响。方法:采用人工合成P0180-199肽段与完全弗氏佐剂混合免疫Lewis大鼠建立EAN模型,1,25(OH)_(2)D_(3)末次灌胃后无菌收集粪便,采用Illumina Miseq PE300型高通量测序仪检测肠道菌群16S rDNA V3-V4可变区,分析肠道菌群结构和丰度变化。结果:1,25(OH)2D3干预可调节EAN大鼠肠道菌群Alpha、Beta多样性,提高菌群丰度、多样性指数。EAN大鼠粪便Verrucomicrobiaceae、Enterobacteriaceae显著增加,Lachnospiraceae、Ruminococcaceae显著减少,均在1,25(OH)_(2)D_(3)干预后回调。结论:1,25(OH)_(2)D_(3)对EAN大鼠肠道菌群有调节作用,肠道微生态的变化可能用于解释EAN的发病机制。
Objective:To explore the intervention effects of active Vitamin D_(3) on gut microbiotal in experimental autoim⁃mune neuritis through 16S rDNA gene sequencing.Methods:EAN model was established by actively immunizing lewis rat with synthetic P0180-199 pepide.The feces were collected aseptically after the last gastric gavage.The fecal DNA was ex⁃tracted and the V3-V4 variable regions of 16S rDNA gene were amplified and sequenced by Illumina Miseq PE300.Gut mi⁃crobial community structure and relative abundance were analyzed using out venn,lefse,etc.Results:1,25(OH)_(2)D_(3) treat⁃ment of EAN rats regulated the alpha and beta diversity index,improved gut microbial diversity.The EAN rats showed a significant increase in Verrucomicrobiaceae,Enterobacteriaeae,and a significant decrease in Lachnospiraceae,Rumino⁃coccaceae,both of them were changed by 1,25(OH)_(2)D_(3).Conclusion:1,25(OH)_(2)D_(3) could regulate the gut microbio⁃ta of EAN rats,and the changes in gut micrbiota,may be used to explain the pathogenesis of EAN.
作者
年娣
孙俊杰
李卓含
张楠
江冉冉
时鹏
NIAN Di;SUN Jun-jie;LI Zhuo-han;ZHANG Nan;JIANG Ran-ran;SHI Peng(Department of Laboratory Medicine,Bengbu Medical College;Bengbu Medical College;Department of Neurology,the First Affiliated Hospital of Bengbu Medical College,Anhui,Bengbu 233000)
出处
《赣南医学院学报》
2021年第10期977-983,1029,共8页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
基金
安徽省教育厅重点项目(KJ2020A0570,KJ2019A0377)
蚌埠医学院大学生创新创业训练计划项目(bydc2020022)。
